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Non-Invasive Model of Neuropathogenic Escherichia coli Infection in the Neonatal Rat

Journal article
Authors F. Dalgakiran
L. A. Witcomb
A. J. McCarthy
George M. H. Birchenough
P. W. Taylor
Published in Jove-Journal of Visualized Experiments
Issue 92
ISSN 1940-087X
Publication year 2014
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Language en
Links dx.doi.org/10.3791/52018
Keywords Infection, Issue 92, Bacterial infection, neonatal bacterial meningitis, bacteremia, sepsis, animal, K1 CAPSULAR POLYSACCHARIDE, BACTERIAL-MENINGITIS, ANTIMICROBIAL, PEPTIDES, INNATE IMMUNITY, NEWBORN RATS, INFANT RATS, EPIDEMIOLOGY, PATHOGENESIS, BACTEREMIA, EXPRESSION, 1E 6BT, England., [Birchenough, George M. H.] Gothenburg Univ, Muc Biol Grp, S-41124 Gothenburg, Sweden., Multidisciplinary Sciences
Subject categories Basic Medicine

Abstract

Investigation of the interactions between animal host and bacterial pathogen is only meaningful if the infection model employed replicates the principal features of the natural infection. This protocol describes procedures for the establishment and evaluation of systemic infection due to neuropathogenic Escherichia coli K1 in the neonatal rat. Colonization of the gastrointestinal tract leads to dissemination of the pathogen along the gut-lymph-blood-brain course of infection and the model displays strong age dependency. A strain of E. coli O18:K1 with enhanced virulence for the neonatal rat produces exceptionally high rates of colonization, translocation to the blood compartment and invasion of the meninges following transit through the choroid plexus. As in the human host, penetration of the central nervous system is accompanied by local inflammation and an invariably lethal outcome. The model is of proven utility for studies of the mechanism of pathogenesis, for evaluation of therapeutic interventions and for assessment of bacterial virulence.

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