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The normal Lactobacillus flora of healthy human rectal and oral mucosa.

Journal article
Authors Siv Ahrné
S Nobaek
B Jeppsson
Ingegerd Adlerberth
Agnes E Wold
G Molin
Published in Journal of applied microbiology
Volume 85
Issue 1
Pages 88-94
ISSN 1364-5072
Publication year 1998
Published at Institute of Laboratory Medicine, Dept of Clinical Immunology
Pages 88-94
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Adult, Bacterial Adhesion, physiology, Biopsy, DNA, Bacterial, genetics, Female, HT29 Cells, physiology, Humans, Intestinal Mucosa, microbiology, Lactobacillus, classification, drug effects, genetics, isolation & purification, pathogenicity, Male, Methylmannosides, pharmacology, Middle Aged, Mouth Mucosa, microbiology, Nucleic Acid Hybridization, genetics, Rectum, microbiology
Subject categories Microbiology, Bacteriology, Gastroenterology and Hepatology

Abstract

The Lactobacillus flora of the rectal and oral mucosa was sampled from 42 healthy volunteers. Species identification was carried out by numerically comparing API 50CH fermentation patterns with type strains, using an SJ-similarity cut-off level of 79%. For the largest groups, identity was further confirmed by DNA-DNA hybridizations against the type strain of the species. Seventeen lactobacilli clusters were defined, of which most were found both on rectal and oral mucosa. The largest taxa were Lactobacillus plantarum, Lact. rhamnosus and Lact. paracasei ssp. paracasei, which were isolated from 52%, 26% and 17% of the individuals, respectively. Most isolates were tested for their capacity to adhere to the human colonic cell line HT-29 in the absence and presence of methyl-alpha-D-mannoside. Mannose-sensitive adherence to HT-29 cells was encountered in two-thirds of the Lact. plantarum isolates, but infrequently among isolates of other taxa. The results suggest that Lact. plantarum is a major colonizer of the human gastrointestinal mucosa, and that its capacity to adhere to mannose-containing receptors may be of some ecological importance.

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