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Incorporating molecular data in fungal systematics: a guide for aspiring researchers

Journal article
Authors Kevin D. Hyde
Dhanushka Udayanga
Dimuthu S. Manamgoda
Leho Tedersoo
Ellen Larsson
Kessy Abarenkov
Yann Bertrand
Bengt Oxelman
Martin Hartmann
Håvard Kauserud
Martin Ryberg
Erik Kristiansson
R. Henrik Nilsson
Published in Current Research in Environmental and Applied Mycology
Volume 3
Issue 1
Pages artikel nr 1
ISSN 2229-2225
Publication year 2013
Published at Department of Mathematical Sciences, Mathematical Statistics
Department of Biological and Environmental Sciences
Pages artikel nr 1
Language en
Keywords biodiversity – molecular marker – phylogeny – publication – species – Sanger sequencing – taxonomy
Subject categories Bioinformatics (Computational Biology), Microbiology, Botany, Bioinformatics and Systems Biology, Terrestrial ecology, Biological Systematics, Evolutionary Biology, Medical microbiology, Soil biology


The last twenty years have witnessed molecular data emerge as a primary research instrument in most branches of mycology. Fungal systematics, taxonomy, and ecology have all seen tremendous progress and have undergone rapid, far-reaching changes as disciplines in the wake of continual improvement in DNA sequencing technology. A taxonomic study that draws from molecular data involves a long series of steps, ranging from taxon sampling through the various laboratory procedures and data analysis to the publication process. All steps are important and influence the results and the way they are perceived by the scientific community. The present paper provides a reflective overview of all major steps in such a project with the purpose to assist research students about to begin their first study using DNA-based methods. We also take the opportunity to discuss the role of taxonomy in biology and the life sciences in general in the light of molecular data. While the best way to learn molecular methods is to work side by side with someone experienced, we hope that the present paper will serve to lower the learning threshold for the reader.

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