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Evidence for an expression of blood group A antigen on platelet glycoproteins IV and V.

Journal article
Authors Dick Stockelberg
M Hou
Lennart Rydberg
Jack Kutti
Hans Wadenvik
Published in Transfusion medicine (Oxford, England)
Volume 6
Issue 3
Pages 243-8
ISSN 0958-7578
Publication year 1996
Published at Institute of Internal Medicine, Dept of Medicine
Institute of Laboratory Medicine, Dept of Clinical Chemistry/Transfusion Medicine
Pages 243-8
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords ABO Blood-Group System, analysis, Adult, Animals, Antibodies, Monoclonal, immunology, Antigens, CD36, chemistry, immunology, Antigens, Human Platelet, chemistry, immunology, Blood Platelets, chemistry, Enzyme-Linked Immunosorbent Assay, Female, Glycosylation, Humans, Infant, Newborn, Mice, Platelet Glycoprotein GPIb-IX Complex, chemistry, immunology, Protein Processing, Post-Translational, Thrombocytopenia, congenital, immunology
Subject categories Clinical Medicine

Abstract

Blood group ABO antigens are known to be carried by several platelet glycoproteins (GP), e.g. GPIb, GPIIa, GPIIb, GPIIa and PECAM. Beside these proteins, we recently observed that blood group A antigen was also expressed on some other uncharacterized platelet proteins (70-90 kDa) having electrophoretic mobilities closely resembling those of GPIV and GPV. These findings prompted us further to characterize these latter ABO-expressing platelet proteins. By antigen capture ELISA, wherein the monoclonal antibodies (mAbs) CLB-IVC7 and CLB-SWI6 were used to hold the corresponding antigens GPIV and GPV, human anti-A specifically bound to these proteins derived from A1-platelets; neither GPIV nor GPV derived from A2-, B- or O-platelets bound anti-A. In a Western blot assay using immunoprecipitated GPIV and GPV as antigens, mAb anti-A immunostained GPIV and GPV precipitated from A1, but not from A2 and O platelets. These results conclusively demonstrate that blood group A antigen is expressed on platelet GPIV and GPV.

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