Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM
| Authors |
Adnan Halim Ulrika Westerlind Christian Pett Manuel Schorlemer Ulla Rüetschi Gunnar Brinkmalm Carina Sihlbom Johan Lengqvist Göran Larson Jonas Nilsson |
|---|---|
| Published in | Journal of proteome research |
| Volume | 13 |
| Issue | 12 |
| Pages | 6024-32 |
| ISSN | 1535-3907 |
| Publication year | 2014 |
| Published at |
Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine Core Facilities, Proteomics Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry |
| Pages | 6024-32 |
| Language | en |
| Links |
dx.doi.org/10.1021/pr500898r |
| Keywords | glycobiology, mass spectrometry, oxonium ions |
| Subject categories | Analytical Chemistry, Bioorganic chemistry, Chemistry, Clinical Laboratory Medicine |
Protein glycosylation plays critical roles in the regulation of diverse biological processes, and determination of glycan structure-function relationships is important to better understand these events. However, characterization of glycan and glycopeptide structural isomers remains challenging and often relies on biosynthetic pathways being conserved. In glycoproteomic analysis with liquid chromatography-tandem mass spectrometry (LC-MS/MS) using collision-induced dissociation (CID), saccharide oxonium ions containing N-acetylhexosamine (HexNAc) residues are prominent. Through analysis of beam-type CID spectra and ion trap CID spectra of synthetic and natively derived N- and O-glycopeptides, we found that the fragmentation patterns of oxonium ions characteristically differ between glycopeptides terminally substituted with GalNAcα1-O-, GlcNAcβ1-O-, Galβ3GalNAcα1-O-, Galβ4GlcNAcβ-O-, and Galβ3GlcNAcβ-O- structures. The difference in the oxonium ion fragmentation profiles of such glycopeptides may thus be used to distinguish among these glycan structures and could be of importance in LC-MS/MS-based glycoproteomic studies.
© UNIVERSITY OF GOTHENBURG • Sweden • Box 100 • SE-405 30 Gothenburg
Phone: +46 31 786 0000 • Contact • Organisation number: 202100-3153
About the website
Facebook
Twitter
Instagram
Youtube
Weibo