To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Sulfatide is associated w… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Sulfatide is associated with insulin granules and located to microdomains of a cultured beta cell line

Journal article
Authors Maria K. Blomqvist
Thomas Osterbye
Jan-Eric Månsson
Thomas Horn
Karsten Buschard
Pam Fredman
Published in Glycoconj J
Volume 19
Issue 6
Pages 403-13
ISSN 0282-0080
Publication year 2002
Published at Institute of Clinical Neurosciences, Section of Experimental Neuroscience
Pages 403-13
Language en
Keywords Animals, Cell Line, Chromatography, Thin Layer, Insulin/*metabolism, Islets of Langerhans/*cytology/*metabolism/ultrastructure, Isotope Labeling, Membrane Microdomains/chemistry/*metabolism, Microscopy, Electron, Rats, Secretory Vesicles/*chemistry/*metabolism, Sulfoglycosphingolipids/*metabolism
Subject categories Neurochemistry


Previous studies using pancreas from various mammals and freshly isolated islets from rat pancreas have provided evidence supporting possible involvement of the glycosphingolipid sulfatide in insulin processing and secretion. In this study, sulfatide expression and metabolism in the beta cell line RINr1046-38 (RIN-38), commonly used as a model for beta cell functional studies, were investigated and compared with previous findings from freshly isolated islets. RIN-38 cells expressed similar amounts (2.7 +/- 1.1 nmol/mg protein, n = 19) of sulfatide as isolated rat islets and also followed the same metabolic pathway, mainly through recycling. Moreover, in agreement with findings in isolated islets, the major species of sulfatide isolated from RIN-38 cells contained C16:0 and C24:0 fatty acids. By applying subcellular isolations and electron microscopy and immunocytochemistry techniques, sulfatide was shown to be located to the secretory granules, the plasma membrane and enriched in detergent insoluble microdomains. In the electron microscopy studies, Sulph I staining was also associated with mitochondria and villi structures. In conclusion, RIN-38 cells might be an appropriate model, as a complement to isolated islets where the amount of material often limits the experiments, to further explore the role of sulfatide in insulin secretion and signal transduction of beta cells.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?