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Angiotensin II receptors, AT1 and AT2 in the rat epididymis. Immunocytochemical and electrophysiological studies.

Journal article
Authors P S Leung
H C Chan
Michael Fu
W L Zhou
P Y Wong
Published in Biochimica et biophysica acta
Volume 1357
Issue 1
Pages 65-72
ISSN 0006-3002
Publication year 1997
Published at Wallenberg Laboratory
Pages 65-72
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Animals, Anions, metabolism, Electrophysiology, Epididymis, metabolism, Fluorescent Antibody Technique, Ion Transport, Male, Rats, Receptor, Angiotensin, Type 1, Receptor, Angiotensin, Type 2, Receptors, Angiotensin, analysis, antagonists & inhibitors, metabolism
Subject categories Physiology, Cell and Molecular Biology

Abstract

Previous work from our laboratory has provided evidence for the presence of a tissue renin-angiotensin system in the rat epididymis. In the current investigation, the regional localization of angiotensin II receptors, type I (AT1) and type II (AT2) was studied immunocytochemically using specific anti-peptide antibodies against the second extracellular loops of AT1 and AT2 receptors, and pharmacologically using specific receptor antagonists in conjunction with the short-circuit current technique. The immunocytochemical results showed that AT1 and AT2 immunoreactivities were predominantly localized in the basal region of the epididymal epithelium. Electrophysiological studies using the short-circuit current technique demonstrated a stimulatory effect of basolaterally applied angiotensin II on the epididymal electrogenic ion transport. This effect was inhibitable by the addition of AT1 antagonist, losartan but not by AT2 antagonist, PD123177, indicating a functional role of AT1 in epididymal electrolyte transport. The present finding suggests that angiotensin II receptors may play an important role in the regulation of epididymal function.

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