To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Fucose-Galactose Polymers… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Fucose-Galactose Polymers Inhibit Cholera Toxin Binding to Fucosylated Structures and Galactose-Dependent Intoxication of Human Enteroids.

Journal article
Authors Jakob Cervin
Andrew Boucher
Gyusaang Youn
Per Björklund
Ville Wallenius
Lynda Mottram
Nicole S Sampson
Ulf Yrlid
Published in ACS infectious diseases
Volume 6
Issue 5
Pages 1192-1203
ISSN 2373-8227
Publication year 2020
Published at Institute of Biomedicine
Institute of Biomedicine, Department of Microbiology and Immunology
Institute of Clinical Sciences, Department of Gastrosurgical Research and Education
Pages 1192-1203
Language en
Keywords cholera toxin, fucose, galactose; inhibition, multivalent glycopolymer, norbornene
Subject categories Basic Medicine, Microbiology in the medical area, Immunology in the medical area


A promising strategy to limit cholera severity involves blockers mimicking the canonical cholera toxin ligand (CT) ganglioside GM1. However, to date the efficacies of most of these blockers have been evaluated in noncellular systems that lack ligands other than GM1. Importantly, the CT B subunit (CTB) has a noncanonical site that binds fucosylated structures, which in contrast to GM1 are highly expressed in the human intestine. Here we evaluate the capacity of norbornene polymers displaying galactose and/or fucose to block CTB binding to immobilized protein-linked glycan structures and also to primary human and murine small intestine epithelial cells (SI ECs). We show that the binding of CTB to human SI ECs is largely dependent on the noncanonical binding site, and interference with the canonical site has a limited effect while the opposite is observed with murine SI ECs. The galactose-fucose polymer blocks binding to fucosylated glycans but not to GM1. However, the preincubation of CT with the galactose-fucose polymer only partially blocks toxic effects on cultured human enteroid cells, while preincubation with GM1 completely blocks CT-mediated secretion. Our results support a model whereby the binding of fucose to the noncanonical site places CT in close proximity to scarcely expressed galactose receptors such as GM1 to enable binding via the canonical site leading to CT internalization and intoxication. Our finding also highlights the importance of complementing CTB binding studies with functional intoxication studies when assessing the efficacy inhibitors of CT.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?