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Unique gene expression patterns indicate microglial contribution to neural stem cell recovery following irradiation.

Journal article
Authors Nina Hellström
Olle R Lindberg
Anders Ståhlberg
John Swanpalmer
Milos Pekny
Klas Blomgren
Hans-Georg Kuhn
Published in Molecular and cellular neurosciences
Volume 46
Issue 4
Pages 710-9
ISSN 1095-9327
Publication year 2011
Published at Institute of Clinical Sciences, Department of Radiation Physics
Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation
Institute of Clinical Sciences, Department of Pediatrics
Pages 710-9
Language en
Links dx.doi.org/10.1016/j.mcn.2011.02.00...
Keywords Animals, Cell Death, drug effects, physiology, Cell Proliferation, drug effects, Cell Survival, Cells, Cultured, Female, Gene Expression, drug effects, radiation effects, Hippocampus, cytology, Humans, Intercellular Signaling Peptides and Proteins, pharmacology, Microglia, cytology, drug effects, physiology, radiation effects, Neural Stem Cells, cytology, drug effects, physiology, radiation effects, Neurogenesis, drug effects, physiology, radiation effects, Polymerase Chain Reaction, methods, Principal Component Analysis, Radiation, Ionizing, Rats, Rats, Wistar
Subject categories Medical and Health Sciences

Abstract

Ionizing radiation results in damage to neural stem cells and reduced neurogenesis. The aim of the present study was to determine intrinsic and extrinsic factors that influence neural stem cell survival following irradiation, using qPCR. Gene expression of hippocampal and SVZ neurospheres were analyzed following irradiation, and results demonstrated that irradiated hippocampal and SVZ stem cells displayed similar gene expression profiles for intrinsic genes. Irradiated microglia (extrinsic factor) isolated from the SVZ exhibited increased gene expression of growth factors involved in stem cell maintenance, proliferation, and survival. However, microglial genes in the irradiated hippocampus responded less favorably with respect to stem cell recovery. This might explain the superior recovery of SVZ compared to hippocampal stem cells following in vivo irradiation. In addition, our results show that a combination of growth factors, which were upregulated in SVZ microglia, increased the proliferation and decreased cell death of irradiated neurospheres in vitro.

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