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The structural basis of blood group A-related glycolipids in an A3 red cell phenotype and potential explanation to a serological phenomenon

Journal article
Authors Lola Svensson
Laura Bindila
Jonas Ångström
Bo Samuelsson
Michael Breimer
Lennart Rydberg
Stephan Henry
Published in Glycobiology
Volume 21
Issue 2
Pages 162-174
ISSN 1460-2423
Publication year 2011
Published at Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine
Institute of Clinical Sciences
Pages 162-174
Language en
Links dx.doi.org/10.1093/glycob/cwq143
Keywords blood group ABO, glycolipid, immunochemistry, mass spectrometry, para-Forssman
Subject categories Chemistry

Abstract

Glycolipids from the red cells of a rare blood group A subgroup individual, expressing the blood group A(3) phenotype with the classical mixed-field agglutination phenomenon, A(2(539G>A))/O(1) genotype, and an unusual blood group A glycolipid profile, were submitted to a comprehensive biochemical and structural analysis. To determine the nature of blood group A glycolipids in this A(3) phenotype, structural determination was carried out with complementary techniques including proton nuclear magnetic resonance (1D and 2D), mass spectrometry (MS) (nano-electrospray ionization/quadrupole time-of-flight and tandem mass spectrometry) and thin layer chromatography with immunostaining detection. As expected, total blood group A structures were of low abundance, but contrary to expectations extended-A type 2 and A type 3 glycolipids were more dominant than A hexaglycosylceramides based on type 2 chain (A-6-2 glycolipids), which normally is the major A glycolipid. Several para-Forssman (GalNAcbeta3GbO(4)) structures, including extended forms, were identified but surmised not to contribute to the classic mixed-field agglutination of the A(3) phenotype. It is proposed that the low level of A antigen combined with an absence of extended branched glycolipids may be the factor determining the mixed-field agglutination phenomenon in this individual.

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