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Effects of nitrogen on growth and carbohydrate formation in Porphyridium cruentum

Journal article
Authors Ali Razaghi
Anna Godhe
Eva Albers
Published in Central European Journal of Biology
Volume 9
Issue 2
Pages 156-162
ISSN 1895-104X
Publication year 2014
Published at Department of Biological and Environmental Sciences
Pages 156-162
Language en
Links dx.doi.org/10.2478/s11535-013-0248-...
https://gup.ub.gu.se/file/127248
Keywords Rhodophyta, Red algae, Redfield ratio, Nitrogen-to-phosphorous ratio, RED ALGA PORPHYRIDIUM, EXTRACELLULAR POLYSACCHARIDE, CHLAMYDOMONAS-REINHARDTI, NUTRIENT LIMITATION, CULTURE-MEDIA, AERUGINEUM, STARVATION, PURPUREUM, CYCLE
Subject categories Biochemistry, Cell Biology, Immunology, Microbiology, Genetics, Phytochemistry including algae and industrial bio-raw materials

Abstract

The microalga Porphyridium cruentum (Rhodophyta) has several industrial and pharmaceutical uses, especially for its polysaccharide production. This study aimed to investigate the influence of nitrogen levels as reflected by altered N:P ratios on the production and content of biomass and carbohydrate. N:P molar ratios were altered in batch cultures to range from 1.6 to 50 using the Redfield ratio of 1:16 as reference. Algal growth (estimated as final cell number, biomass concentration and maximum specific growth rate) was negatively affected at low N:P ratios. The optimal N:P ratio for growth was identified at 35-50, with specific growth rates of 0.19 day(-1) and maximum cell concentrations of 59 center dot 10(8) cells L-1 and 1.2 g dry weight of biomass L-1. In addition, variation in cell size was seen. Cells with larger diameters were at higher N:P ratios and smaller cells at lower ratios. The cellular carbohydrate content increased under reduced nitrogen availability. However, because accumulation was moderate at the lowest N:P ratio, 0.4 g per g dry weight biomass compared to 0.24 at the Redfield ratio of 16:1, conditions for increased total carbohydrate formation were identified at the N:P ratios optimal for growth. Additionally, carbohydrates were largely accumulated in late exponential to stationary phase.

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