To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Mitochondrial transcripti… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Mitochondrial transcription termination factor 1 directs polar replication fork pausing

Journal article
Authors Yonghong Shi
Viktor Posse
Xuefeng Zhu
A. K. Hyvarinen
H. T. Jacobs
Maria Falkenberg
Claes M Gustafsson
Published in Nucleic Acids Research
Volume 44
Issue 12
Pages 5732-5742
ISSN 0305-1048
Publication year 2016
Published at Institute of Biomedicine
Pages 5732-5742
Language en
Keywords dna-polymerase-gamma, in-vitro, binding protein, rna-polymerase, ribosomal-rna, factor mterf, lagging-strand, human mtdna, twinkle, cells, Biochemistry & Molecular Biology
Subject categories Medical Biotechnology


During replication of nuclear ribosomal DNA (rDNA), clashes with the transcription apparatus can cause replication fork collapse and genomic instability. To avoid this problem, a replication fork barrier protein is situated downstream of rDNA, there preventing replication in the direction opposite rDNA transcription. A potential candidate for a similar function in mitochondria is the mitochondrial transcription termination factor 1 (MTERF1, also denoted mTERF), which binds to a sequence just downstream of the ribosomal transcription unit. Previous studies have shown that MTERF1 prevents antisense transcription over the ribosomal RNA genes, a process which we here show to be independent of the transcription elongation factor TEFM. Importantly, we now demonstrate that MTERF1 arrests mitochondrial DNA (mtDNA) replication with distinct polarity. The effect is explained by the ability of MTERF1 to act as a directional contrahelicase, blocking mtDNA unwinding by the mitochondrial helicase TWINKLE. This conclusion is also supported by in vivo evidence that MTERF1 stimulates TWINKLE pausing. We conclude that MTERF1 can direct polar replication fork arrest in mammalian mitochondria.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?