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The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting glycan microarray-based data

Journal article
Authors Y. Liu
R. McBride
M. Stoll
A. S. Palma
L. Silva
S. Agravat
K. F. Aoki-Kinoshita
M. P. Campbell
C. E. Costello
A. Dell
S. M. Haslam
Niclas G. Karlsson
K. H. Khoo
D. Kolarich
M. V. Novotny
N. H. Packer
R. Ranzinger
E. Rapp
P. M. Rudd
W. B. Struwe
M. Tiemeyer
L. Wells
W. S. York
J. Zaia
C. Kettner
J. C. Paulson
T. Feizi
D. F. Smith
Published in Glycobiology
Volume 27
Issue 4
Pages 280-284
ISSN 0959-6658
Publication year 2017
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 280-284
Language en
Links 10.1093/glycob/cww118
Keywords glycan microarrays, glycans, glycobiology, glycomics, MIRAGE, shotgun glycomics, mass-spectrometry, binding-proteins, experiment, miame, array data, receptors, oligosaccharides, neoglycolipids, specificities, biomarkers, B 440, S-40530 Gothenburg, Sweden., [Khoo, Kay-Hooi] Acad Sinica, Inst Biol Chem, 128,Acad Rd Sec 2, Taipei 115, Taiwan., [Kolarich, Daniel] Max Planck Inst Colloids & Interfaces, Dept Biomol Syst, D-14424 Potsdam, Germany., [Novotny, Milos V.] Indiana Univ, Dept Chem, 800 E Kirkwood Ave, Bloomington, IN 47405 USA., [Ranzinger, Rene, Tiemeyer, Michael, Wells, Lance, York, William S.] Univ Georgia, Complex, [Rapp, Erdmann] Max Planck Inst Dynam Complex Tech Syst, Bioproc Engn, D-39106 Magdeburg,, [Rudd, Pauline M.] NIBRT Natl Inst Bioproc Res & Training, NIBRT GlycoSci Grp, Fosters Ave, Blackrock,, [Struwe, Weston B.] Univ Oxford, Glycobiol Inst, Dept Biochem, Oxford OX1 3QU, England., [Kettner, Carsten] Beilstein Inst, Trakehner Str 7-9, D-60487 Frankfurt, Germany., [Smith, David F.] Emory Univ, Sch Med, Emory Comprehens Glyc Core, Atlanta, GA 30322 USA., ng pw, 1985, biochemical and biophysical research communications, v132, p474
Subject categories Cell and Molecular Biology


MIRAGE (Minimum Information Required for A Glycomics Experiment) is an initiative that was created by experts in the fields of glycobiology, glycoanalytics and glycoinformatics to produce guidelines for reporting results from the diverse types of experiments and analyses used in structural and functional studies of glycans in the scientific literature. As a sequel to the guidelines for sample preparation (Struwe et al. 2016, Glycobiology, 26: 907-910) and mass spectrometry data (Kolarich et al. 2013, Mol. Cell Proteomics, 12: 991-995), here we present the first version of guidelines intended to improve the standards for reporting data from glycan microarray analyses. For each of eight areas in the workflow of a glycan microarray experiment, we provide guidelines for the minimal information that should be provided in reporting results. We hope that the MIRAGE glycan microarray guidelines proposed here will gain broad acceptance by the community, and will facilitate interpretation and reproducibility of the glycan microarray results with implications in comparison of data from different laboratories and eventual deposition of glycan microarray data in international databases.

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