To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Mapping Ribonucleotides I… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Mapping Ribonucleotides Incorporated into DNA by Hydrolytic End-Sequencing.

Journal article
Authors Clinton D Orebaugh
Scott A Lujan
Adam B Burkholder
Anders R Clausen
Thomas A Kunkel
Published in Methods in molecular biology (Clifton, N.J.)
Volume 1672
Pages 329-345
ISSN 1940-6029
Publication year 2018
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 329-345
Language en
Keywords Computational Biology, methods, DNA, isolation & purification, metabolism, DNA Repair, DNA Replication, DNA, Fungal, isolation & purification, Gene Library, Genomics, methods, High-Throughput Nucleotide Sequencing, Hydrolysis, Ribonuclease H, metabolism, Ribonucleotides, Yeasts
Subject categories Genetics, Functional genomics, Molecular biology


Ribonucleotides embedded within DNA render the DNA sensitive to the formation of single-stranded breaks under alkali conditions. Here, we describe a next-generation sequencing method called hydrolytic end sequencing (HydEn-seq) to map ribonucleotides inserted into the genome of Saccharomyce cerevisiae strains deficient in ribonucleotide excision repair. We use this method to map several genomic features in wild-type and replicase variant yeast strains.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?