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GsSLAH3, a Glycine soja slow type anion channel homolog, positively modulates plant bicarbonate stress tolerance

Journal article
Authors X. B. Duan
Y. Yu
H. Duanmu
C. Chen
X. L. Sun
L. Cao
Q. Li
X. D. Ding
Liu Beidong
Y. M. Zhu
Published in Physiologia Plantarum
Volume 164
Issue 2
Pages 145-162
ISSN 0031-9317
Publication year 2018
Published at Department of Chemistry and Molecular Biology
Pages 145-162
Language en
Keywords arabidopsis-thaliana, alkaline stress, gene-expression, schizosaccharomyces-pombe, abiotic stresses, aba sensitivity, salt, tolerance, sulfite efflux, guard-cells, nitrate
Subject categories Plant Biotechnology


Alkaline stress is a major form of abiotic stress that severely inhibits plant growth and development, thus restricting crop productivity. However, little is known about how plants respond to alkali. In this study, a slow-type anion channel homolog 3 gene, GsSLAH3, was isolated and functionally characterized. Bioinformatics analysis showed that the GsSLAH3 protein contains 10 transmembrane helices. Consistently, GsSLAH3 was found to locate on plasma membrane by transient expression in onion epidermal cells. In wild soybeans, GsSLAH3 expression was induced by NaHCO3 treatment, suggesting its involvement in plant response to alkaline stress. Ectopic expression of GsSLAH3 in yeast increased sensitivity to alkali treatment. Dramatically, overexpression of GsSLAH3 in Arabidopsis thaliana enhanced alkaline tolerance during the germination, seedling and adult stages. More interestingly, we found that transgenic lines also improved plant tolerance to KHCO3 rather than high pH treatment. A nitrate content analysis of Arabidopsis shoots showed that GsSLAH3 overexpressing lines accumulated more NO3- than wild-type. In summary, our data suggest that GsSLAH3 is a positive alkali responsive gene that increases bicarbonate resistance specifically.

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