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Chromophore-Protein Interplay during the Phytochrome Photocycle Revealed by Step-Scan FTIR Spectroscopy

Journal article
Authors J. A. Ihalainen
Emil Gustavsson
L. Schroeder
S. Donnini
H. Lehtivuori
Linnéa Isaksson
C. Thoing
V. Modi
Oskar Berntsson
B. Stucki-Buchli
A. Liukkonen
H. Hakkanen
E. Kalenius
S. Westenhoff
T. Kottke
Published in Journal of the American Chemical Society
Volume 140
Issue 39
Pages 12396-12404
ISSN 0002-7863
Publication year 2018
Published at Department of Chemistry and Molecular Biology
Pages 12396-12404
Language en
Links dx.doi.org/10.1021/jacs.8b04659
Keywords time-resolved ftir, photoactive yellow protein, induced proton release, structural-changes, crystal-structure, ir spectroscopy, raman-spectroscopy, nmr-spectroscopy, oat phytochrome, ground-state, Chemistry, ates of america, v106, p6123, ates of america, v106, p1784, ates of america, v105, p14709, ates of america, v104, p5383, ates of america, v111, p3871, ates of america, v110, pe1273
Subject categories Chemistry

Abstract

Phytochrome proteins regulate many photo-responses of plants and microorganisms. Light absorption causes isomerization of the biliverdin chromophore, which triggers a series of structural changes to activate the signaling domains of the protein. However, the structural changes are elusive, and therefore the molecular mechanism of signal transduction remains poorly understood. Here, we apply twocolor step-scan infrared spectroscopy to the bacteriophytochrome from Deinococcus radiodurans. We show by recordings in H2O and D2O that the hydrogen bonds to the biliverdin D-ring carbonyl become disordered in the first intermediate (Lumi-R) forming a dynamic microenvironment, then completely detach in the second intermediate (Meta-R), and finally reform in the signaling state (Pfr). The spectra reveal via isotope labeling that the refolding of the conserved "PHY-tongue" region occurs with the last transition between Meta-R and Pfr. Additional changes in the protein backbone are detected already within microseconds in Lumi-R Aided by molecular dynamics simulations, we find that a strictly conserved salt bridge between an arginine of the PHY tongue and an aspartate of the chromophore binding domains is broken in Lumi-R and the arginine is recruited to the D-ring C=O. This rationalizes how isomerization of the chromophore is linked to the global structural rearrangement in the sensory receptor. Our findings advance the structural understanding of phytochrome photoactivation.

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