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Methodological Issues in Studying PAHSA Biology: Masking PAHSA Effects.

Journal article
Authors Ismail Syed
Jennifer Lee
Odile D Peroni
Mark M Yore
Pedro M Moraes-Vieira
Anna Santoro
Kerry Wellenstein
Ulf Smith
Timothy E McGraw
Alan Saghatelian
Barbara B Kahn
Published in Cell metabolism
Volume 28
Issue 4
Pages 543-546
ISSN 1932-7420
Publication year 2018
Published at Institute of Medicine, Department of Molecular and Clinical Medicine
Pages 543-546
Language en
Links dx.doi.org/10.1016/j.cmet.2018.09.0...
www.ncbi.nlm.nih.gov/entrez/query.f...
Subject categories Basic Medicine, Clinical Medicine, Biological Sciences

Abstract

PAHSAs are anti-diabetic and anti-inflammatory lipids. Syed et al. identify numerous experimental differences that likely account for the failure of Pflimlin et al. to observe PAHSA beneficial effects. The differences include different HFDs resulting in minimal/no glucose intolerance, different assay conditions, an LC-MS protocol that was not validated, and use of olive oil, a bioactive nutrient that improves glucose tolerance, as a vehicle.

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