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FRAP: A Powerful Method to Evaluate Membrane Fluidity in Caenorhabditis elegans

Journal article
Authors Ranjan Devkota
Marc Pilon
Published in Bio-Protocol
Volume 8
Issue 13
Publication year 2018
Published at Department of Chemistry and Molecular Biology
Language en
Links dx.doi.org/10.21769/BioProtoc.2913
Keywords FRAP, Membrane fluidity, Prenylated GFP, C. elegans, Fatty acid, PAQR-2, Life Sciences & Biomedicine - Other Topics
Subject categories Molecular biology

Abstract

FRAP (Fluorescence Recovery After Photobleaching) is probably the most direct method to investigate the dynamics of molecules in living cells. Here, we describe FRAP to quantify membrane fluidity in C. elegans. Using FRAP, we have shown that cold, glucose and exogenous saturated fatty acids can decrease the fluidity of cellular membranes in certain mutants.

Page Manager: Webmaster|Last update: 9/11/2012
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