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Vagococcus bubulae sp. nov., isolated from ground beef, and Vagococcus vulneris sp. nov., isolated from a human foot wound

Journal article
Authors P. L. Shewmaker
A. M. Whitney
C. A. Gulvik
B. Humrighouse
J. Gartin
H. Moura
J. R. Barr
Edward R.B. Moore
Roger Karlsson
T. C. A. Pinto
L. M. Teixeira
Published in International Journal of Systematic and Evolutionary Microbiology
Volume 69
Issue 8
Pages 2268-2276
ISSN 1466-5026
Publication year 2019
Published at Institute of Biomedicine, Department of Infectious Medicine
Pages 2268-2276
Language en
Links dx.doi.org/10.1099/ijsem.0.003459
Keywords taxonomy, Firmicutes, Vagococcus, Vagococcus bubulae, Vagococcus vulneris, gram-positive cocci, gene, identification, enterococcus, classification, streptococcus, algorithm, bacteria, strains, rpob, Microbiology
Subject categories Infectious Medicine

Abstract

Two unusual catalase-negative, Gram-stain-positive, Vagococcus-like isolates that were referred to the CDC Streptococcus Laboratory for identification are described. Strain SS1994(T) was isolated from ground beef and strain SS1995(T) was isolated from a human foot wound. Comparative 16S rRNA gene sequence analysis of isolates SS1994(T) and SS1995(T) against Vagococcus type strain sequences supported their inclusion in the genus Vagococcus. Strain SS1994(T) showed high sequence similarity (>97.0 %) to the two most recently proposed species, Vagococcus martis (99.2 %) and Vagococcus teuberi (99.0 %) followed by Vagococcus penaei (98.8 %), strain SS1995(T) (98.6 %), Vagococcus camiphilus (98.0 %), Vagococcus acidifermentans (98.0 %) and Vagococcus fluvialis (97.9 %). The 16S rRNA gene sequence of strain SS1995(T) was most similar to V. penaei (99.1 %), followed by SS1994(T) (98.6%), V. martis (98.4 %), V. teuberi (98.1 %), V. acidifermentans (97.8 %), and both V. camiphilus and V. fluvialis (97.5 %). A polyphasic taxonomic study using conventional biochemical and the rapid ID 32 STREP system, MALDI-TOF MS, cell fatty acid analysis, pairwise sequence comparisons of the 16S rRNA, rpoA, rpoB, pheS and groL genes, and comparative core and whole genome sequence analyses revealed that strains SS1994(T) and SS1995(T) were two novel Vagococcus species. The novel taxonomic status of the two isolates was confirmed with core genome phylogeny, average nucleotide identity <84 %and in silico DNA-DNA hybridization <28 %to any other Vagococcus species. The names Vagococcus bubulae SS1994 (T)=(CCUG 70831(T) =LMG 30164(T)) and Vagococcus vulneris SS1995(T)=(CCUG 70832(T) =LMG 30165(T)) are proposed.

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