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Plasticity in exocytosis revealed through the effects of repetitive stimuli affect the content of nanometer vesicles and the fraction of transmitter released

Journal article
Authors Chaoyi Gu
Anna Larsson
Andrew G Ewing
Published in Proceedings of the National Academy of Sciences of the United States of America
Volume 116
Issue 43
Pages 21409-21415
ISSN 0027-8424
Publication year 2019
Published at Department of Chemistry and Molecular Biology
Pages 21409-21415
Language en
Links dx.doi.org/10.1073/pnas.1910859116
Keywords amperometry, vesicle impact electrochemical cytometry, exocytosis, repetitive stimuli, plasticity, protein-kinase-c, activity-dependent potentiation, catecholamine, release, quantal size, fusion pore, chromaffin, cells, pheochromocytoma, activation, dynamin, Science & Technology - Other Topics
Subject categories Electrochemistry

Abstract

Electrochemical techniques with disk and nano-tip electrodes, together with calcium imaging, were used to examine the effect of short-interval repetitive stimuli on both exocytosis and vesicular content in a model cell line. We show that the number of events decreases markedly with repeated stimuli suggesting a depletion of exocytosis machinery. However, repetitive stimuli induce a more stable fusion pore, leading to an increased amount of neurotransmitter release. In contrast, the total neurotransmitter content inside the vesicles decreases after repetitive stimuli, resulting in a higher average release fraction from each event. We suggest a possible mechanism regarding a link between activity-induced plasticity and fraction of release.

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