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| Authors |
Ganesh V Shelke Yanan Yin Su Chul Jang Cecilia Lässer S. Wennmalm H. J. Hoffmann L. Li Y. S. Gho Jonas Andreas Nilsson Jan Lötvall |
|---|---|
| Published in | Journal of Extracellular Vesicles |
| Volume | 8 |
| Issue | 1 |
| Publication year | 2019 |
| Published at |
Institute of Clinical Sciences, Department of Surgery Krefting Research Centre |
| Language | en |
| Links |
dx.doi.org/10.1080/20013078.2019.16... |
| Keywords | Mast cells, extracellular vesicles, exosomes, mesenchymal stem cells, tumour growth factor beta-1, cellular localization, endosomal signalling, proteoglycan, mesenchymal stem-cells, heparan-sulfate proteoglycans, growth-factor-beta, tgf-beta, mediated transfer, cancer exosomes, internalization, fibroblast, phenotype, migration, Cell Biology, ipale j, 1994, journal of cell biology, v124, p171 |
| Subject categories | Cell biology |
Extracellular vesicles such as exosomes convey biological messages between cells, either by surface-to-surface interaction or by shuttling of bioactive molecules to a recipient cell's cytoplasm. Here we show that exosomes released by mast cells harbour both active and latent transforming growth factor beta-1 (TGF beta-1) on their surfaces. The latent form of TGF beta-1 is associated with the exosomes via heparinase-II and pH-sensitive elements. These vesicles traffic to the endocytic compartment of recipient human mesenchymal stem cells (MSCs) within 60 min of exposure. Further, the exosomes-associated TGF beta-1 is retained within the endosomal compartments at the time of signalling, which results in prolonged cellular signalling compared to free-TGF beta-1. These exosomes induce a migratory phenotype in primary MSCs involving SMAD-dependent pathways. Our results show that mast cell-derived exosomes are decorated with latent TGF beta-1 and are retained in recipient MSC endosomes, influencing recipient cell migratory phenotype. We conclude that exosomes can convey signalling within endosomes by delivering bioactive surface ligands to this intracellular compartment.
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