To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Dinucleotide Degradation … - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Dinucleotide Degradation by REXO2 Maintains Promoter Specificity in Mammalian Mitochondria

Journal article
Authors Thomas J. Nicholls
H. Spahr
S. Jiang
S. J. Siira
C. Koolmeister
S. Sharma
J. H. K. Kauppila
M. Jiang
V. Kaever
O. Rackham
A. Chabes
Maria Falkenberg
A. Filipovska
N. G. Larsson
Claes M Gustafsson
Published in Molecular Cell
Volume 76
Issue 5
Pages 784-+
ISSN 1097-2765
Publication year 2019
Published at Institute of Biomedicine
Pages 784-+
Language en
Links dx.doi.org/10.1016/j.molcel.2019.09...
Keywords escherichia-coli, transcription initiation, stranded-rna, in-vitro, oligoribonuclease, polymerase, DNA, maintenance, component, model, Biochemistry & Molecular Biology, Cell Biology
Subject categories Cell and molecular biology

Abstract

Oligoribonucleases are conserved enzymes that degrade short RNA molecules of up to 5 nt in length and are assumed to constitute the final stage of RNA turnover. Here we demonstrate that REXO2 is a specialized dinucleotide-degrading enzyme that shows no preference between RNA and DNA dinucleotide substrates. A heart- and skeletal-muscle-specific knockout mouse displays elevated dinucleotide levels and alterations in gene expression patterns indicative of aberrant dinucleotide-primed transcription initiation. We find that dinucleotides act as potent stimulators of mitochondrial transcription initiation in vitro. Our data demonstrate that increased levels of dinucleotides can be used to initiate transcription, leading to an increase in transcription levels from both mitochondrial promoters and other, nonspecific sequence elements in mitochondrial DNA. Efficient RNA turnover by REXO2 is thus required to maintain promoter specificity and proper regulation of transcription in mammalian mitochondria.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?