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Changes in epigenetic pattern in osteoblasts in response to surface characteristics.

Authors Yuki Ichioka
Farah Asa'ad
Behnosh Öhrnell Malekzadeh
Anna Westerlund
Lena Larsson
Published in Virtual Osteology Symposium USA
Publication year 2020
Published at Institute of Odontology
Institute of Clinical Sciences, Department of Biomaterials
Institute of Odontology, Section 3
Language en
Keywords titanium, osteoblast, DNA damage, DNA repair, epigenetics
Subject categories Biomaterials Science, Medical Biotechnology, Biomaterials


Objective: The overall aim of the current project was to investigate the influence of titanium and titanium surface characteristics on epigenetic mechanisms. Materials & Methods: An osteoblast-like cells (MG63) was incubated on glass, smooth titanium and rough titanium respectively for 0,1,6 and 24 h. The presence of double stranded DNA damage, DNA repair and epigenetic markers were investigated using immunofluorescence. Results: The percentage of total Chk2 positive cells, which indicate DNA repair, was 0% on the rough titanium surfaces at all time points, in comparison to glass and smooth titanium. Regarding DNA damage, total -H2AX positive cells on the rough titanium gradually decreased as incubation time increased, on the contrary to smooth titanium. For epigenetic markers related to the DNA damage/repair pathway, rough titanium surface showed the lower percentage of AcH3 positive cells compared to glass and smooth titanium surface. Conclusions: The findings in the current study show that titanium surface characteristics indeed influence DNA damage and the DNA repair pathway, including epigenetic factors.

Page Manager: Webmaster|Last update: 9/11/2012

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