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Localization of cholesterol, phosphocholine and galactosylceramide in rat cerebellar cortex with imaging TOF-SIMS equipped with a bismuth cluster ion source.

Journal article
Authors Håkan Nygren
Katrin Börner
Birgit Hagenhoff
Per Malmberg
Jan-Eric Månsson
Published in Biochimica et biophysica acta
Volume 1737
Issue 2-3
Pages 102-10
ISSN 0006-3002
Publication year 2005
Published at Institute of Anatomy and Cell Biology
Institute of Clinical Neurosciences, Section of Experimental Neuroscience
Pages 102-10
Language en
Links dx.doi.org/10.1016/j.bbalip.2005.10...
Keywords Animals, Bismuth, Cerebellar Cortex, metabolism, Cholesterol, metabolism, Galactosylceramides, metabolism, Male, Phosphorylcholine, metabolism, Purkinje Cells, metabolism, Rats, Rats, Sprague-Dawley, Spectrometry, Mass, Secondary Ion, methods, Tissue Distribution
Subject categories Medical and Health Sciences

Abstract

Time-of-flight secondary-ion-mass-spectrometry (TOF-SIMS) was utilized to address the issue of co-localization of cholesterol, phosphocholine and galactosylceramide in rat cerebellar cortex. Rat cerebellum was fixed, freeze-protected by sucrose, frozen and sectioned by cryoultramicrotomy and dried at room temperature. The samples were analyzed in an imaging TOF-SIMS instrument equipped with a Bi(1-7)+-source. The cholesterol signal (m/z 369 and 385) was localized in Purkinje cells and in nuclei of granular layer cells. The phosphocholine headgroup of phosphatidylcholine and sphingomyelin was localized by imaging a specific fragment (m/z 86). This signal was localized in the molecular layer of cerebellar cortex, in Purkinje cells and in parts of the granular layer probably representing the synapse-rich glomeruli. The galactosylceramide was localized by imaging the quasi-molecular ions at m/z 835 and 851, showed a clear colocalization with cholesterol, but also a specific localization in dots (diameter

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