Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Expression of scavenger r… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Kontaktformulär








 


OBS! Vill du ha svar, ange e-post eller telefonnummer!




Expression of scavenger receptor class B type I in gallbladder columnar epithelium.

Artikel i vetenskaplig tidskrift
Författare Magnus S.C. Johnson
Per-Arne Svensson
Jan Borén
Håkan Billig
Lena M S Carlsson
Björn Carlsson
Publicerad i Journal of gastroenterology and hepatology
Volym 17
Nummer/häfte 6
Sidor 713-20
ISSN 0815-9319
Publiceringsår 2002
Publicerad vid Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi
Sidor 713-20
Språk en
Länkar doi.org/10.1046/j.1440-1746.2002.02...
Ämnesord Animals, Antigens, CD36, metabolism, Apolipoprotein B-100, Apolipoproteins B, metabolism, Blotting, Western, Cholesterol, LDL, blood, Cholesterol, VLDL, blood, Epithelium, metabolism, Gallbladder, metabolism, Immunohistochemistry, Membrane Proteins, Mice, Mice, Transgenic, Receptors, Immunologic, Receptors, Lipoprotein, Receptors, Scavenger, Reverse Transcriptase Polymerase Chain Reaction, Scavenger Receptors, Class B
Ämneskategorier Medicinska grundvetenskaper

Sammanfattning

The lipid content of bile may be modified by the gallbladder epithelium. Recent studies indicate that cholesterol can be absorbed from bile and that this can be enhanced by apolipoprotein (apo) A-I. SR-BI is a multifunctional receptor capable of binding a wide array of native or modified lipoproteins, phospholipid or bile acid micelles. As apo A-I is a ligand for scavenger receptor class B type I (SR-BI) we have characterized the expression of this receptor in murine gallbladder.Reverse transcription-polymerase chain reaction (RT-PCR), immunoblotting and immunohistochemistry were used to study SR-BI expression in murine gallbladders. SR-BI expression was also used to examine gallbladders from high-fat-fed wild-type and apo B-100 transgenic mice.SR-BI and SR-BII mRNA are expressed in gallbladder. SR-BI immunoreactivity was localized to the columnar epithelium of the gallbladder. Immunoreactive SR-BI in gallbladder had an estimated molecular weight of 57 kDa, in contrast to the expected 82 kDa. Deglycosylation experiments indicated that the size difference between the two forms of the receptor is due to post-translational modification. Fat feeding of apo B transgenic mice resulted in gallstone formation but had no effect on the abundance of SR-BI.Gallbladder epithelial cells express SR-BI. This opens the possibility that SR-BI may influence the modification of bile in the gallbladder.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?