Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Concentration-Induced Ass… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Concentration-Induced Association in a Protein System Caused by a Highly Directional Patch Attraction

Artikel i vetenskaplig tidskrift
Författare Weimin Li
Björn A. Persson
Mikael Lund
Johan Bergenholtz
Malin Zackrisson Oskolkova
Publicerad i Journal of Physical Chemistry B
Volym 120
Sidor 8953-8959
ISSN 1520-6106
Publiceringsår 2016
Publicerad vid Institutionen för kemi och molekylärbiologi
Sidor 8953-8959
Språk en
Länkar pubs.acs.org/doi/abs/10.1021/acs.jp...
Ämnesord protein interactions, SAXS, light scattering, integral equation theory, structure factor
Ämneskategorier Biofysikalisk kemi, Yt- och kolloidkemi, Statistisk mekanik

Sammanfattning

Self-association of the protein lactoferrin is studied in solution using small-angle X-ray scattering techniques. Effective static structure factors have been shown to exhibit either a monotonic or a nonmonotonic dependence on protein concentration in the small wavevector limit, depending on salt concentration. The behavior correlates with a nonmonotonic dependence of the second virial coefficient on salt concentration, such that a maximum appears in the structure factor at a low protein concentration when the second virial coefficient is negative and close to a minimum. The results are interpreted in terms of an integral equation theory with explicit dimers, formulated by Wertheim, which provides a consistent framework able to explain the behavior in terms of a monomer–dimer equilibrium that appears because of a highly directional patch attraction. Short attraction ranges preclude trimer formation, which explains why the protein system behaves as if it were subject to a concentration-dependent isotropic protein–protein attraction. Superimposing an isotropic interaction, comprising screened Coulomb repulsion and van der Waals attraction, on the patch attraction allows for a semiquantitative modeling of the complete transition pathway from monomers in the dilute limit to monomer–dimer systems at somewhat higher protein concentrations.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?

Denna text är utskriven från följande webbsida:
http://gu.se/forskning/publikation/?publicationId=241419
Utskriftsdatum: 2020-03-31