Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

A novel conformation of t… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

A novel conformation of the herpes simplex virus origin of DNA replication recognized by the origin binding protein.

Artikel i vetenskaplig tidskrift
Författare Alireza Aslani
Stina Simonsson
Per Elias
Publicerad i The Journal of biological chemistry
Volym 275
Nummer/häfte 8
Sidor 5880-7
ISSN 0021-9258
Publiceringsår 2000
Publicerad vid Institutionen för medicinsk och fysiologisk kemi
Sidor 5880-7
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Base Sequence, DNA Replication, genetics, DNA, Single-Stranded, metabolism, DNA, Viral, chemistry, DNA-Binding Proteins, metabolism, Electrophoresis, Agar Gel, Escherichia coli, metabolism, Models, Genetic, Molecular Sequence Data, Mutation, Nucleic Acid Conformation, Oligonucleotides, metabolism, Plasmids, Replication Origin, genetics, Simplexvirus, chemistry, genetics, Single-Strand Specific DNA and RNA Endonucleases, metabolism, Time Factors, Viral Proteins, metabolism
Ämneskategorier Virologi, Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

Sammanfattning

The Herpes simplex virus type I origin binding protein (OBP) is a sequence-specific DNA-binding protein and a dimeric DNA helicase encoded by the UL9 gene. It is required for the activation of the viral origin of DNA replication oriS. Here we demonstrate that the linear double-stranded form of oriS can be converted by heat treatment to a stable novel conformation referred to as oriS*. Studies using S1 nuclease suggest that oriS* consists of a central hairpin with an AT-rich sequence in the loop. Single-stranded oligonucleotides corresponding to the upper strand of oriS can adopt the same structure. OBP forms a stable complex with oriS*. We have identified structural features of oriS* recognized by OBP. The central oriS palindrome as well as sequences at the 5' side of the oriS palindrome were required for complex formation. Importantly, we found that mutations that have been shown to reduce oriS-dependent DNA replication also reduce the formation of the OBP-oriS* complex. We suggest that oriS* serves as an intermediate in the initiation of DNA replication providing the initiator protein with structural information for a selective and efficient assembly of the viral replication machinery.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?