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Flow cytometric analysis of megakaryocyte ploidy in chronic myeloproliferative disorders and reactive thrombocytosis.

Artikel i vetenskaplig tidskrift
Författare Stefan Jacobsson
Jan Carneskog
Börje Ridell
Hans Wadenvik
Birgitta Swolin
Jack Kutti
Publicerad i European journal of haematology
Volym 56
Nummer/häfte 5
Sidor 287-92
ISSN 0902-4441
Publiceringsår 1996
Publicerad vid Institutionen för laboratoriemedicin , Avdelningen för patologi
Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin
Institutionen för invärtesmedicin, Avdelningen för internmedicin
Sidor 287-92
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Adult, Aged, Female, Flow Cytometry, methods, Humans, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, blood, genetics, Male, Megakaryocytes, pathology, Middle Aged, Myeloproliferative Disorders, blood, genetics, Platelet Count, Ploidies, Polycythemia Vera, blood, genetics, Reference Values, Thrombocytosis, blood, etiology, genetics
Ämneskategorier Tumörbiologi, Patologi, Hematologi, Klinisk kemi

Sammanfattning

Megakaryocyte (MK) ploidy patterns were analysed by flow cytometry in 29 newly diagnosed and previously untreated patients with chronic myeloproliferative disorders (MPD) and concomitant thrombocytosis, in 9 patients with reactive thrombocytosis (RT) and in 12 healthy individuals. Unfractionated bone marrow from routine aspirates was used. MKs were identified with a fluorescein labelled monoclonal antibody specific for glycoprotein IIIa (GPIIIa) and DNA was stained with propidium iodide. For the 12 healthy volunteers the mean modal ploidy number was 16 N; the 9 patients with RT displayed an identical MK ploidy pattern. The frequency of MKs with a ploidy > or = 32 N was 45% among the patients with essential thrombocythaemia (ET) compared to 32% among the healthy volunteers (p < 0.001). MKs with ploidy number > or = 64 N, comprising approximately 13% of the total number of MKs, was a characteristic finding in the patients with ET. Similar findings were present in 8 patients with polycythaemia vera (PV). In patients with PV 34% and 6% of the MKs displayed ploidies > or = 32 N and > or = 64 N, respectively. In contrast, a distinct shift towards lower ploidy number, with 63% of MKs < or = 8 N, was found among the 4 patients with chronic myeloid leukaemia (CML). The present results indicate that by using flow cytometric analysis of MK ploidy distribution in patients with thrombocytosis, those with a reactive cause are likely to be discriminated from patients with myeloproliferative thrombocytosis, i.e. PV and ET on one hand and CML on the other hand. The distinction between ET and PV, however, has to be made on other grounds.

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