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Light chain-restricted autoantibodies in chronic idiopathic thrombocytopenic purpura, but no evidence for circulating clone B-lymphocytes.

Artikel i vetenskaplig tidskrift
Författare Dick Stockelberg
M Hou
Stefan Jacobsson
Jack Kutti
Hans Wadenvik
Publicerad i Annals of hematology
Volym 72
Nummer/häfte 1
Sidor 29-34
ISSN 0939-5555
Publiceringsår 1996
Publicerad vid Institutionen för invärtesmedicin, Avdelningen för internmedicin
Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin
Sidor 29-34
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Adolescent, Adult, Aged, Autoantibodies, blood, immunology, B-Lymphocytes, immunology, Base Sequence, Chronic Disease, Enzyme-Linked Immunosorbent Assay, Female, Humans, Immunoglobulin Light Chains, immunology, Lymphocyte Subsets, Male, Middle Aged, Molecular Sequence Data, Purpura, Thrombocytopenic, Idiopathic, blood, immunology
Ämneskategorier Tumörbiologi, Hematologi, Klinisk kemi

Sammanfattning

In chronic idiopathic thrombocytopenic purpura (ITP) platelet destruction is caused by antibodies directed against platelet membrane glycoproteins (GP), and the predominant autoantigens are known to be GPIb/IX and GPIIb/IIIa. In a recent study we reported that these antibodies frequently had a restricted light chain phenotype, thereby supporting a clonal origin. Similar findings and the presence of clonal B-cell populations in immune thrombocytopenias have been reported by others. In the present study we further explored the hypothesis of clonal B-cell expansions in chronic ITP. Twenty patients with chronic ITP were investigated. Antibodies were detected with an ELISA (MAIPA) specific for GPIb/IX and GPIIb/IIIa; circulating clonal B lymphocytes were assessed by flow-cytometric (FACS) clonal-excess analysis and by analyzing Ig-gene rearrangements (CDR3) with the PCR technique. Nine patients displayed a GP-specific antibody restricted to either kappa or lambda phenotype. However, FACS analysis and Ig-gene rearrangement studies did not disclose any circulating clonal B-cell population. Considering the sensitivity of the FACs analysis and Ig-gene rearrangement for detection of clonal B-cell populations, the hypothesis of clonally derived autoantibodies in ITP is still valid. Most probably, the clonal B-cell expansion responsible for the production of autoantibodies in ITP, if present, is below the detection limit for the techniques employed.

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