Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

The salivary mucin MG1 (M… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

The salivary mucin MG1 (MUC5B) carries a repertoire of unique oligosaccharides that is large and diverse.

Artikel i vetenskaplig tidskrift
Författare Kristina A Thomsson
Akraporn Prakobphol
Hakon Leffler
Molakala S Reddy
Michael J Levine
Susan J Fisher
Gunnar C. Hansson
Publicerad i Glycobiology
Volym 12
Nummer/häfte 1
Sidor 1-14
ISSN 0959-6658
Publiceringsår 2002
Publicerad vid Institutionen för medicinsk och fysiologisk kemi
Sidor 1-14
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Carbohydrate Sequence, Humans, Molecular Sequence Data, Mucin-5B, Mucins, chemistry, Nuclear Magnetic Resonance, Biomolecular, Oligosaccharides, chemistry, Saliva, chemistry, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Ämneskategorier Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

Sammanfattning

The high-molecular-mass salivary mucin MG1, one of two major mucins produced by human salivary glands, plays an important role in oral health by coating the tooth surface and by acting as a bacterial receptor. Here this mucin was purified from the submandibular/sublingual saliva of a blood group O individual. The presence of MUC5B as the major mucin in this preparation was confirmed by amino acid analysis and its reactivity with the monoclonal antibody PAN H2. To structurally characterize MG1 carbohydrates the O-glycans were released by reductive beta-elimination. Nuclear magnetic resonance spectroscopy of the nonfractionated mixture showed that (1) fucose was present in blood group H, Le(a), Le(x), Le(b), and Le(y) epitopes; (2) NeuAc was mainly linked alpha 2-3 to Gal or alpha 2-6 to GalNAcol; and (3) the major internal structures were core 1 and core 2 sequences. After this preliminary analysis the released oligosaccharides were separated into neutral (56%), sialylated (26%), and sulfated (19%) fractions, with an average length of 13, 17, and 41 sugar residues, respectively. Gas chromatography-mass spectrometry and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of mixtures of neutral and sialylated oligosaccharides revealed at least 62 neutral and 25 sialylated oligosaccharides consisting of up to 20 monosaccharide residues. These results showed that the MG1-derived oligosaccharides were much longer than those of MG2, and only a few species were found on both molecules. Thus, these two mucins create an enormous repertoire of potential binding sites for microorganisms at one of the major portals where infectious organisms enter the body.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?