Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

The prolyl 3-hydroxylases… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

The prolyl 3-hydroxylases P3H2 and P3H3 are novel targets for epigenetic silencing in breast cancer.

Artikel i vetenskaplig tidskrift
Författare R Shah
P Smith
C Purdie
P Quinlan
L Baker
Pierre Åman
A M Thompson
T Crook
Publicerad i British journal of cancer
Volym 100
Nummer/häfte 10
Sidor 1687-96
ISSN 1532-1827
Publiceringsår 2009
Publicerad vid Institutionen för biomedicin, avdelningen för patologi
Sidor 1687-96
Språk en
Länkar dx.doi.org/10.1038/sj.bjc.6605042
Ämnesord Breast Neoplasms, genetics, Carcinoma, genetics, Cell Line, Tumor, Cells, Cultured, CpG Islands, genetics, DNA Methylation, physiology, Down-Regulation, Epigenesis, Genetic, physiology, Female, Gene Expression Regulation, Neoplastic, Gene Silencing, Genes, Tumor Suppressor, physiology, Humans, Procollagen-Proline Dioxygenase, genetics, physiology, Receptors, Estrogen, genetics, metabolism, Tumor Stem Cell Assay
Ämneskategorier Cell- och molekylärbiologi, Cancer och onkologi, Dermatologi och venereologi

Sammanfattning

Expression of P3H2 (Leprel1) and P3H3 (Leprel2) but not P3H1 (Leprecan) is down-regulated in breast cancer by aberrant CpG methylation in the 5' regulatory sequences of each gene. Methylation of P3H2 appears specific to breast cancer as no methylation was detected in a range of cell lines from other epithelial cancers or from primary brain tumours or malignant melanoma. Methylation in P3H2, but not P3H3, was strongly associated with oestrogen-receptor-positive breast cancers, whereas methylation in P3H3 was associated with higher tumour grade and Nottingham Prognostic Index. Ectopic expression of P3H2 and P3H3 in cell lines with silencing of the endogenous gene results in suppression of colony growth. This is the first demonstration of epigenetic inactivation of prolyl hydroxylases in human cancer, implying that this gene family represents a novel class of tumour suppressors. The restriction of silencing in P3H2 to breast carcinomas, and its association with oestrogen-receptor-positive cases, suggests that P3H2 may be a breast-cancer-specific tumour suppressor.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?