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Mapping of a functional autoimmune epitope on the beta 1-adrenergic receptor in patients with idiopathic dilated cardiomyopathy.

Artikel i vetenskaplig tidskrift
Författare Yvonne Magnusson
S Marullo
S Hoyer
Finn Waagstein
Bert Andersson
A Vahlne
J G Guillet
A D Strosberg
Agneta Hjalmarson
Johan Hoebeke
Publicerad i The Journal of clinical investigation
Volym 86
Nummer/häfte 5
Sidor 1658-63
ISSN 0021-9738
Publiceringsår 1990
Publicerad vid Medicinska institutionen
Sidor 1658-63
Språk en
Länkar dx.doi.org/10.1172/JCI114888
Ämnesord Adult, Aged, Amino Acid Sequence, Antibody Affinity, Autoantibodies, blood, immunology, Autoimmune Diseases, immunology, Cardiomyopathy, Dilated, immunology, Epitopes, immunology, Female, Humans, Immunoenzyme Techniques, Male, Middle Aged, Molecular Sequence Data, Receptors, Adrenergic, beta, chemistry, immunology
Ämneskategorier Cell- och molekylärbiologi

Sammanfattning

The presence and properties of serum autoantibodies against beta-adrenergic receptors in patients with idiopathic dilated cardiomyopathy were studied using synthetic peptides derived from the predicted sequences of the human beta-adrenergic receptors. Peptides corresponding to the sequences of the second extracellular loop of the human beta 1- and beta 2-adrenergic receptors were used as antigens in an enzyme immunoassay to screen sera from patients with dilated cardiomyopathy (n = 42), ischemic heart disease (n = 17), or healthy blood donors (n = 34). The sera of thirteen dilated cardiomyopathy patients, none of the ischemic heart disease patients, and four of the healthy controls monospecifically recognized the beta 1-peptide. Only affinity-purified antibodies of these patients had a inhibitory effect on radioligand binding to the beta 1 receptor of C6 rat glioma cells. They recognized the receptor protein by immunoblot and bound in situ to human myocardial tissue. We conclude that a subgroup of patients with idiopathic dilated cardiomyopathy have in their sera autoantibodies specifically directed against the second extracellular loop of the beta 1-adrenergic receptor. These antibodies could serve as a marker of an autoimmune response with physiological and/or pathological implications.

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