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A truncated form of HpaA is a promising antigen for use in a vaccine against Helicobacter pylori.

Artikel i vetenskaplig tidskrift
Författare Carl-Fredrik Flach
Natascha Svensson
Margareta Blomquist
Annelie Ekman
Sukanya Raghavan
Jan Holmgren
Publicerad i Vaccine
Volym 29
Nummer/häfte 6
Sidor 1235-1241
ISSN 1873-2518
Publiceringsår 2011
Publicerad vid Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi
Sidor 1235-1241
Språk en
Länkar dx.doi.org/10.1016/j.vaccine.2010.1...
Ämnesord HpaA; Vaccine; Helicobacter pylori
Ämneskategorier Medicinsk mikrobiologi, Immunologi inom det medicinska området, Immunbiologi

Sammanfattning

HpaA is a Helicobacter pylori-specific lipoprotein that has been shown to be an effective protective antigen for mucosal vaccination against H. pylori infection in mice. However, detergents are needed for the purification of full-length HpaA (HpaA(full)), which might confer toxicity, thus making HpaA(full) unsuitable for use in a human vaccine. We here describe a recombinantly produced truncated version of HpaA (HpaA(trunc)), which is easily purified without the use of detergents. Evaluation in the murine H. pylori infection model showed that sublingual immunization with HpaA(trunc) was equally immunogenic and protective as immunization with HpaA(full). Immunization with a combination of HpaA(trunc) and recombinant UreB protein induced strong immune responses to both antigens and importantly had a strong synergistic effect on protection, associated with synergistically increased expression of IL-17 in the stomach. Notably, sublingual immunization with HpaA(trunc) and UreB was superior to corresponding intragastric immunization with regard to the level of protection induced. In conclusion, HpaA(trunc) is a promising, readily produced, non-toxic recombinant antigen for inclusion in a mucosal vaccine against H. pylori infection, which may preferably be given sublingually together with UreB.

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