Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Minimizing tissue damage … - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Minimizing tissue damage in electroosmotic sampling.

Artikel i vetenskaplig tidskrift
Författare Amy E Hamsher
Hongjuan Xu
Yifat Guy
Mats Sandberg
Stephen G Weber
Publicerad i Analytical chemistry
Volym 82
Nummer/häfte 15
Sidor 6370-6
ISSN 1520-6882
Publiceringsår 2010
Publicerad vid Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor 6370-6
Språk en
Länkar dx.doi.org/10.1021/ac101271r
Ämnesord Animals, Apoptosis, Cell Death, Electroosmosis, methods, Hippocampus, injuries, pathology, Organ Culture Techniques, Propidium, chemistry, Rats, Rats, Sprague-Dawley, Silicon Dioxide, chemistry
Ämneskategorier Neurokemi, Kemi

Sammanfattning

Electroosmotic sampling is a potentially powerful method for pulling extracellular fluid into a fused-silica capillary in contact with the surface of tissue. An electric field is created in tissue by passing current through an electrolyte-filled capillary and then through the tissue. The resulting field acts on the counterions to the surface charges in the extracellular space to create electroosmotic fluid flow within the extracellular space of a tissue. Part of the development of this approach is to define conditions under which electroosmotic sampling minimizes damage to the tissue, in this case organotypic hippocampal slice cultures (OHSCs). We have assessed tissue damage by measuring fluorescence resulting from exposing sampled tissue to propidium iodide solution 16-24 h after sampling. Sampling has been carried out with a variety of capillary diameters, capillary tip-tissue distances, and applied voltages. Tissue damage is negligible when the power (current x potential drop) created in the tissue is less than 120 microW. In practical terms, smaller capillary i.d.s, lower voltages, and greater tissue to capillary distances lead to lower power.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?