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Presence of IGF-I in human midgut carcinoid tumours--an autocrine regulator of carcinoid tumour growth?

Artikel i vetenskaplig tidskrift
Författare Ola Nilsson
Bo Wängberg
E Theodorsson
A Skottner
Håkan Ahlman
Publicerad i International journal of cancer. Journal international du cancer
Volym 51
Nummer/häfte 2
Sidor 195-203
ISSN 0020-7136
Publiceringsår 1992
Publicerad vid Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi
Sidor 195-203
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Adult, Aged, Carcinoid Tumor, chemistry, secretion, Chromatography, High Pressure Liquid, DNA, analysis, Female, Humans, Ileal Neoplasms, chemistry, secretion, Immunohistochemistry, Insulin-Like Growth Factor I, analysis, genetics, secretion, Male, Middle Aged, Receptors, Cell Surface, analysis, genetics, Receptors, Somatomedin
Ämneskategorier Cancer och onkologi, Kirurgi

Sammanfattning

The presence of IGF-I and IGF-I receptors in human midgut carcinoid tumours has been investigated. Using immunocytochemistry, IGF-I-positive tumour cells were demonstrated in 11/11 tumour cases studied. Labelling of consecutive sections with antibodies against IGF-I and proliferating cell nuclear antigen (PCNA)/cyclin demonstrated a co-distribution of the 2 antigens in carcinoid tumours. Extracts of tumour tissues were subjected to radioimmunoassay and shown to contain significant amounts of IGF-I. Reverse-phase HPLC of tumour extracts demonstrated a major IGF-I-immunoreactive component eluting in the position of rhIGF-I, but also 2 other more hydrophobic forms. Conditioned serum-free media from primary cultures of carcinoid tumors contained detectable amounts of IGF-I, indicating a spontaneous release of IGF-I from tumour cells into the culture medium. Levels of IGF-I in media were reduced (19%) after incubation of cultures with a somatostatin analogue for 4 days. IGF-I receptors were observed on tumour cells in 4/10 tumours by immunocytochemistry. Tumour cells with immunoreactive IGF-I receptors could be stimulated to enhanced growth, measured as an increase in DNA contents, by exogenous administration of IGF-I every 3-4 days for 2 weeks. The results show that cultured human midgut carcinoid tumours secrete IGF-I and that some of the tumours also have IGF-I receptors. We therefore suggest that IGF-I may act as an autocrine or paracrine regulator of carcinoid tumour-cell growth.

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