Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Glycoproteins with Gal al… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Glycoproteins with Gal alpha 4Gal are absent from human erythrocyte membranes, indicating that glycolipids are the sole carriers of blood group P activities.

Artikel i vetenskaplig tidskrift
Författare Z Yang
Jörgen Bergström
Karl-Anders Karlsson
Publicerad i The Journal of biological chemistry
Volym 269
Nummer/häfte 20
Sidor 14620-4
ISSN 0021-9258
Publiceringsår 1994
Publicerad vid Institutionen för medicinsk och fysiologisk kemi
Sidor 14620-4
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Antibodies, Monoclonal, Binding Sites, Antibody, Carbohydrate Conformation, Carbohydrate Sequence, Electrophoresis, Polyacrylamide Gel, Erythrocyte Membrane, chemistry, Glycolipids, metabolism, Humans, Membrane Glycoproteins, blood, chemistry, isolation & purification, Molecular Sequence Data, Oligosaccharides, analysis, chemistry, P Blood-Group System, metabolism, Recombinant Proteins, chemistry, isolation & purification, metabolism
Ämneskategorier Kemi

Sammanfattning

The antigenic determinants of the blood group P family (P1, P, Pk, and LKE antigens) are chemically based on Gal alpha 4Gal. For human erythrocytes it has been claimed that the majority of P1 determinants are expressed in glycoproteins, mainly band 4.5 (Haselberger, C. G., and Schenkel-Brunner, H. (1982) FEBS Lett. 149, 126-128). In the present work, the existence of Gal alpha 4Gal in glycoproteins of erythrocyte membranes (ghosts) of P1 positive and negative human individuals was carefully analyzed on replicas of sodium dodecyl sulfate-polyacrylamide electrophoresis gels using specific reagents (Escherichia coli HB101/pDC1 expressing the Pap gene and monoclonal antibodies with specificities for P1 and Pk antigens). No binding to glycoproteins was detected with any of these ligands when the ghosts had been pretreated with butanol to remove glycolipids. Therefore, all antigenic determinants of the P blood group family on human red cells are exclusively expressed in glycolipids and are absent from glycoproteins.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?