Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän

Development of bright flu… - Göteborgs universitet Till startsida
Till innehåll Läs mer om hur kakor används på gu.se

Development of bright fluorescent quadracyclic adenine analogues: TDDFT-calculation supported rational design

Artikel i vetenskaplig tidskrift
Författare Anders Foller Larsen
Blaise Dumat
Moa Sandberg Wranne
Christopher P. Lawson
S. Preus
Mattias Bood
Henrik Gradén
Marcus Wilhelmsson
Morten Grøtli
Publicerad i Scientific Reports
Volym 5
Sidor art. no. 12653
ISSN 2045-2322
Publiceringsår 2015
Publicerad vid Institutionen för kemi och molekylärbiologi
Sidor art. no. 12653
Språk en
Länkar dx.doi.org/10.1038/srep12653
Ämnesord Nucleic-Acid, Photophysical Properties, Cytosine Analog, Base Analog, Pyrrolo-Dc, Nucleoside, Density, Oligonucleotides, Probes, Fluorophore
Ämneskategorier Biokemi och molekylärbiologi, Fysikalisk kemi, Biofysikalisk kemi, Spektroskopi, Bioorganisk kemi, Fysikalisk organisk kemi


Fluorescent base analogues (FBAs) comprise a family of increasingly important molecules for the investigation of nucleic acid structure and dynamics. We recently reported the quantum chemical calculation supported development of four microenvironment sensitive analogues of the quadracyclic adenine (qA) scaffold, the qANs, with highly promising absorptive and fluorescence properties that were very well predicted by TDDFT calculations. Herein, we report on the efficient synthesis, experimental and theoretical characterization of nine novel quadracyclic adenine derivatives. The brightest derivative, 2-CNqA, displays a 13-fold increased brightness (epsilon Phi(F) = 4500) compared with the parent compound qA and has the additional benefit of being a virtually microenvironment-insensitive fluorophore, making it a suitable candidate for nucleic acid incorporation and use in quantitative FRET and anisotropy experiments. TDDFT calculations, conducted on the nine novel qAs a posteriori, successfully describe the relative fluorescence quantum yield and brightness of all qA derivatives. This observation suggests that the TDDFT-based rational design strategy may be employed for the development of bright fluorophores built up from a common scaffold to reduce the otherwise costly and time-consuming screening process usually required to obtain useful and bright FBAs.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?