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Human adipose tissue expresses angiotensinogen and enzymes required for its conversion to angiotensin II.

Artikel i vetenskaplig tidskrift
Författare C Karlsson
Kajsa Lindell
Malin Ottosson
Lars Sjöström
Björn Carlsson
Lena M S Carlsson
Publicerad i The Journal of clinical endocrinology and metabolism
Volym 83
Nummer/häfte 11
Sidor 3925-9
ISSN 0021-972X
Publiceringsår 1998
Publicerad vid Institutionen för invärtesmedicin
Sidor 3925-9
Språk en
Länkar dx.doi.org/10.1210/jcem.83.11.5276
Ämnesord Adipose Tissue, enzymology, metabolism, Adult, Angiotensin II, metabolism, Angiotensinogen, biosynthesis, metabolism, Cathepsin D, metabolism, Cathepsin G, Cathepsins, metabolism, Female, Humans, Middle Aged, Peptidyl-Dipeptidase A, genetics, metabolism, RNA, Messenger, analysis, Renin, genetics, Renin-Angiotensin System, physiology, Reverse Transcriptase Polymerase Chain Reaction, Serine Endopeptidases, metabolism
Ämneskategorier Cell- och molekylärbiologi

Sammanfattning

Angiotensin II regulates blood pressure and may affect adipogenesis and adipocyte metabolism. Angiotensin II is produced by cleavage of angiotensinogen by renin and angiotensin-converting enzyme in the circulation. In addition, angiotensin II may be produced in various tissues by enzymes of the renin-angiotensin system (RAS) or the nonrenin-angiotensin system (NRAS). We have analyzed the expression of angiotensinogen and enzymes required for its conversion to angiotensin II in human adipose tissue. Northern blot demonstrated angiotensinogen expression in adipose tissue from nine obese subjects. Western blot revealed a distinct band of expected size of the angiotensinogen protein (61 kDa) in isolated adipocytes. RT-PCR, followed by Southern blot, demonstrated renin expression in human adipose tissue. Angiotensin-converting enzyme messenger RNA was detected by RT-PCR, and the identity of the PCR products was verified by restriction enzyme cleavage. Transcripts for cathepsin D and cathepsin G, components of the NRAS, were detected by RT-PCR, verified by restriction enzyme cleavage. We conclude that human adipose tissue expresses angiotensinogen and enzymes of RAS and NRAS. This opens the possibility that angiotensinogen-derived peptides, produced in adipose tissue itself, may affect adipogenesis and play a role in the pathogenesis of obesity.

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