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Interplay between flow and diffusion in capillary alginate hydrogels

Artikel i vetenskaplig tidskrift
Författare Erich Schuster
Kristin Sott
Anna Ström
Annika Altskar
N. Smisdom
Tobias Gebäck
Niklas Lorén
Anne-Marie Hermansson
Publicerad i Soft Matter
Volym 12
Nummer/häfte 17
Sidor 3897-3907
ISSN 1744-683X
Publiceringsår 2016
Publicerad vid Institutionen för matematiska vetenskaper, matematik
Sidor 3897-3907
Språk en
Länkar dx.doi.org/10.1039/c6sm00294c
Ämnesord laser-scanning microscope, correlation spectroscopy, fluorescence, recovery, membrane dynamics, gels, scaffolds, parameters, models, Chemistry, Materials Science, Physics, Polymer Science
Ämneskategorier Fysik, Polymerkemi, Materialkemi

Sammanfattning

Alginate gels with naturally occurring macroscopic capillaries have been used as a model system to study the interplay between laminar flow and diffusion of nanometer-sized solutes in real time. Calcium alginate gels that contain homogeneously distributed parallel-aligned capillary structures were formed by external addition of crosslinking ions to an alginate sol. The effects of different flow rates (0, 1, 10, 50 and 100 mu l min(-1)) and three different probes (fluorescein, 10 kDa and 500 kDa fluorescein isothiocyanate-dextran) on the diffusion rates of the solutes across the capillary wall and in the bulk gel in between the capillaries were investigated using confocal laser scanning microscopy. The flow in the capillaries was produced using a syringe pump that was connected to the capillaries via a tube. Transmission electron microscopy revealed an open aggregated structure close to the capillary wall, followed by an aligned network layer and the isotropic network of the bulk gel. The most pronounced effect was observed for the 1 nm-diameter fluorescein probe, for which an increase in flow rate increased the mobility of the probe in the gel. Fluorescence recovery after photobleaching confirmed increased mobility close to the channel, with increasing flow rate. Mobility maps derived using raster image correlation spectroscopy showed that the layer with the lowest mobility corresponded to the anisotropic layer of ordered network chains. The combination of microscopy techniques used in the present study elucidates the flow and diffusion behaviors visually, qualitatively and quantitatively, and represents a promising tool for future studies of mass transport in non-equilibrium systems.

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