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Concentration-Induced Association in a Protein System Caused by a Highly Directional Patch Attraction

Artikel i vetenskaplig tidskrift
Författare Weimin Li
Björn A. Persson
Mikael Lund
Johan Bergenholtz
Malin Zackrisson Oskolkova
Publicerad i Journal of Physical Chemistry B
Volym 120
Sidor 8953-8959
ISSN 1520-6106
Publiceringsår 2016
Publicerad vid Institutionen för kemi och molekylärbiologi
Sidor 8953-8959
Språk en
Länkar pubs.acs.org/doi/abs/10.1021/acs.jp...
Ämnesord protein interactions, SAXS, light scattering, integral equation theory, structure factor
Ämneskategorier Biofysikalisk kemi, Yt- och kolloidkemi, Statistisk mekanik

Sammanfattning

Self-association of the protein lactoferrin is studied in solution using small-angle X-ray scattering techniques. Effective static structure factors have been shown to exhibit either a monotonic or a nonmonotonic dependence on protein concentration in the small wavevector limit, depending on salt concentration. The behavior correlates with a nonmonotonic dependence of the second virial coefficient on salt concentration, such that a maximum appears in the structure factor at a low protein concentration when the second virial coefficient is negative and close to a minimum. The results are interpreted in terms of an integral equation theory with explicit dimers, formulated by Wertheim, which provides a consistent framework able to explain the behavior in terms of a monomer–dimer equilibrium that appears because of a highly directional patch attraction. Short attraction ranges preclude trimer formation, which explains why the protein system behaves as if it were subject to a concentration-dependent isotropic protein–protein attraction. Superimposing an isotropic interaction, comprising screened Coulomb repulsion and van der Waals attraction, on the patch attraction allows for a semiquantitative modeling of the complete transition pathway from monomers in the dilute limit to monomer–dimer systems at somewhat higher protein concentrations.

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