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The phagocyte chemiluminescence paradox: luminol can act as an inhibitor of neutrophil NADPH-oxidase activity.

Artikel i vetenskaplig tidskrift
Författare Jenny Fäldt
Malin Ridell
Anna Karlsson
Claes Dahlgren
Publicerad i Luminescence : the journal of biological and chemical luminescence
Volym 14
Nummer/häfte 3
Sidor 153-60
ISSN 1522-7235
Publiceringsår 1999
Publicerad vid Institutionen för medicinsk mikrobiologi och immunologi
Sidor 153-60
Språk en
Länkar dx.doi.org/10.1002/(SICI)1522-7243(...
www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Cell-Free System, Enzyme Inhibitors, pharmacology, Humans, In Vitro Techniques, Kinetics, Luminescent Measurements, Luminol, analogs & derivatives, pharmacology, N-Formylmethionine Leucyl-Phenylalanine, pharmacology, NADPH Oxidase, antagonists & inhibitors, Phagocytes, drug effects, enzymology, Signal Transduction, drug effects, Superoxides, metabolism, Tetradecanoylphorbol Acetate, pharmacology
Ämneskategorier Mikrobiologi inom det medicinska området, Immunologi inom det medicinska området

Sammanfattning

The chemiluminescence system amplified by luminol or isoluminol is a sensitive and widely used method for determination of respiratory burst products generated by the NADPH-oxidase in phagocytes. The present study shows that luminol, but not isoluminol, can inhibit the release of oxygen metabolites generated by human neutrophil NADPH-oxidase. The difference in structure between luminol and isoluminol (rendering luminol more lipophilic than isoluminol, and thereby membrane-permeable), is suggested to determine indirectly whether or not the molecule is inhibitory. Luminol was shown to have an increased inhibitory effect after preincubation of neutrophils on a surface of aggregated IgG, suggesting that the cells can be transferred from a 'luminol-insensitive' to a 'luminol-sensitive' state. Since luminol had no inhibitory effect in a cell-free NADPH-oxidase system, it is likely that it interferes with the signal transduction pathway, leading to assembly and/or activation of the oxidase. As a consequence of the present results, showing that luminol but not isoluminol can inhibit NADPH-oxidase activity, we suggest that isoluminol is used in future studies of superoxide anion release from phagocytes.

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