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The lysosomal membrane glycoproteins Lamp-1 and Lamp-2 are present in mobilizable organelles, but are absent from the azurophil granules of human neutrophils.

Artikel i vetenskaplig tidskrift
Författare Claes Dahlgren
S R Carlsson
Anna Karlsson
H Lundqvist
Publicerad i The Biochemical journal
Volym 311 ( Pt 2)
Sidor 667-74
ISSN 0264-6021
Publiceringsår 1995
Publicerad vid Institutionen för invärtesmedicin, Avdelningen för reumatologi och inflammationsforskning
Institutionen för medicinsk mikrobiologi och immunologi
Sidor 667-74
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Antibodies, Monoclonal, Antigens, CD, metabolism, Blotting, Western, Cell Membrane, metabolism, Cytoplasmic Granules, metabolism, Electrophoresis, Polyacrylamide Gel, Flow Cytometry, Fluorescent Antibody Technique, Humans, Lysosome-Associated Membrane Glycoproteins, Membrane Glycoproteins, metabolism, Neutrophils, metabolism, ultrastructure, Organelles, metabolism, Phagocytosis, physiology, Receptors, Complement, metabolism, Subcellular Fractions, metabolism, Up-Regulation
Ämneskategorier Mikrobiologi inom det medicinska området, Immunologi inom det medicinska området

Sammanfattning

The subcellular localization of two members of a highly glycosylated protein group present in lysosomal membranes in most cells, the lysosome-associated membrane proteins 1 and 2 (Lamp-1 and Lamp-2), was examined in human neutrophil granulocytes. Antibodies that were raised against purified Lamp-1 adn Lamp-2 gave a distinct granular staining of the cytoplasm upon immunostaining of neutrophils. Subcellular fractionation was used to separate the azurophil and specific granules from a light-membrane fraction containing plasma membranes and secretory vesicles, and Western blotting was used to determine the presence of the Lamps in these fractions. The results show that Lamp-1 and Lamp-2 are present in the specific-granule-enriched fraction and in the light-membrane fraction, but not in the azurophil granules. Separation of secretory vesicles from plasma membranes disclosed that the light-membrane Lamps were present primarily in the secretory-vesicle-enriched fraction. During phagocytosis both Lamp-1 and Lamp-2 became markedly concentrated around the ingested particle and they both appear on the cell surface when the secretory organelles are mobilized.

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