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Delivery of cyclodextrin polymers to bacterial biofilms - An exploratory study using rhodamine labelled cyclodextrins and multiphoton microscopy

Artikel i vetenskaplig tidskrift
Författare Hanna Thomsen
Gábor Benkovics
Éva Fenyvesi
Anne Farewell
Milo Malanga
Marica B Ericson
Publicerad i International Journal of Pharmaceutics
Volym 531
Nummer/häfte 2
Sidor 650-657
ISSN 0378-5173
Publiceringsår 2017
Publicerad vid Institutionen för kemi och molekylärbiologi
CARe - Centrum för antibiotikaresistensforskning
Sidor 650-657
Språk en
Länkar https://doi.org/10.1016/j.ijpharm.2...
Ämnesord Charge functionalization, Cyclodextrin polymers, Multiphoton laser scanning microscopy, Rhodamine, Spectral analysis, Staphylococcus epidermidis biofilm
Ämneskategorier Nanoteknik, Mikrobiologi, Biofysikalisk kemi

Sammanfattning

© 2017 The Authors. Cyclodextrin (CD) polymers are interesting nanoparticulate systems for pharmaceutical delivery; however, knowledge regarding their applications towards delivery into complex microbial biofilm structures is so far limited. The challenge is to demonstrate penetration and transport through the biofilm and its exopolysaccharide matrix. The ideal functionalization for penetration into mature biofilms is unexplored. In this paper, we present a novel set of rhodamine labelled βCD-polymers, with different charge moieties, i.e., neutral, anionic, and cationic, and explore their potential delivery into mature Staphylococcus epidermidis biofilms using multiphoton laser scanning microscopy (MPM). The S. epidermidis biofilms, being a medically relevant model organism, were stained with SYTO9. By using MPM, three-dimensional imaging and spectral investigation of the distribution of the βCD-polymers could be obtained. It was found that the cationic βCD-polymers showed significantly higher integration into the biofilms, compared to neutral and anionic functionalized βCDs. None of the carriers presented any inherent toxicity to the biofilms, meaning that the addition of rhodamine moiety does not affect the inertness of the delivery system. Taken together, this study demonstrates a novel approach by which delivery of fluorescently labelled CD nanoparticles to bacterial biofilms can be explored using MPM. Future studies should be undertaken investigating the potential in using cationic functionalization of CD based delivery systems for targeting anti-microbial effects in biofilms.

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