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D-methionine interferes with non-typeable Haemophilus influenzae peptidoglycan synthesis during growth and biofilm formation

Artikel i vetenskaplig tidskrift
Författare H. Dawe
Evelin Berger
Carina Sihlbom
E. M. Angus
R. P. Howlin
J. R. Laver
M. Tebruegge
L. Hall-Stoodley
P. Stoodley
S. N. Faust
R. N. Allan
Publicerad i Microbiology-Sgm
Volym 163
Nummer/häfte 7
Sidor 1093-1104
ISSN 1350-0872
Publiceringsår 2017
Publicerad vid Core Facilities, Proteomics
Sidor 1093-1104
Språk en
Länkar doi.org/10.1099/mic.0.000491
Ämnesord Haemophilus influenzae, biofilm, proteomics, methionine, peptidoglycan, d-amino acids, escherichia-coli, cell-wall, bacterial, gene, children, resistance, tolerance, infection, division, Microbiology
Ämneskategorier Mikrobiologi


Non-typeable Haemophilus influenzae (NTHi) is an opportunistic pathogen that plays a major role in a number of respiratory tract infections, including otitis media, cystic fibrosis and chronic obstructive pulmonary disease. Biofilm formation has been implicated in both NTHi colonization and disease, and is responsible for the increased tolerance of this pathogen towards antibiotic treatment. Targeting metabolic pathways that are important in NTHi biofilm formation represents a potential strategy to combat this antibiotic recalcitrance. A previous investigation demonstrated increased expression of a putative D-methionine uptake protein following exposure of NTHi biofilms to the ubiquitous signalling molecule, nitric oxide. We therefore hypothesized that treatment with exogenous D-methionine would impact on NTHi biofilm formation and increase antibiotic sensitivity. Treatment of NTHi during the process of biofilm formation resulted in a reduction in biofilm viability, increased biomass, changes in the overall biofilm architecture and the adoption of an amorphous cellular morphology. Quantitative proteomic analyses identified 124 proteins that were differentially expressed following D-methionine treatment, of which 51 (41 %) were involved in metabolic and transport processes. Nine proteins involved in peptidoglycan synthesis and cell division showed significantly increased expression. Furthermore, D-methionine treatment augmented the efficacy of azithromycin treatment and highlighted the potential of D-methionine as an adjunctive therapeutic approach for NTHi biofilm-associated infections.

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