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Stem Cell-Derived Exosomes as Nanotherapeutics for Autoimmune and Neurodegenerative Disorders

Artikel i vetenskaplig tidskrift
Författare M. Riazifar
M. R. Mohammadi
E. J. Pone
A. Yeri
Cecilia Lässer
A. I. Segaliny
L. L. McIntyre
Ganesh V Shelke
E. Hutchins
A. Hamamoto
E. N. Calle
Rossella Crescitelli
W. Liao
V. Pham
Y. Yin
J. Jayaraman
J. R. T. Lakey
C. M. Walsh
K. Van Keuren-Jensen
Jan Lötvall
W. Zhao
Publicerad i ACS Nano
Volym 13
Nummer/häfte 6
Sidor 6670-6688
ISSN 1936-0851
Publiceringsår 2019
Publicerad vid Institutionen för kliniska vetenskaper, Avdelningen för kirurgi
Krefting Research Centre
Sidor 6670-6688
Språk en
Länkar dx.doi.org/10.1021/acsnano.9b01004
Ämnesord drug delivery, exosomes, extracellular vesicles, mesenchymal stem cells, multiple sclerosis, nanotherapeutics, regulatory T cells, Controlled drug delivery, Flowcharting, Mammals, Neurodegenerative diseases, Nucleic acids, Stem cells, T-cells, Extracellular, Mesenchymal stem cell, Cell culture
Ämneskategorier Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

Sammanfattning

To dissect therapeutic mechanisms of transplanted stem cells and develop exosome-based nanotherapeutics in treating autoimmune and neurodegenerative diseases, we assessed the effect of exosomes secreted from human mesenchymal stem cells (MSCs) in treating multiple sclerosis using an experimental autoimmune encephalomyelitis (EAE) mouse model. We found that intravenous administration of exosomes produced by MSCs stimulated by IFN (IFN-Exo) (i) reduced the mean clinical score of EAE mice compared to PBS control, (ii) reduced demyelination, (iii) decreased neuroinflammation, and (iv) upregulated the number of CD4+CD25+FOXP3+ regulatory T cells (Tregs) within the spinal cords of EAE mice. Co-culture of IFN-Exo with activated peripheral blood mononuclear cells (PBMCs) cells in vitro reduced PBMC proliferation and levels of pro-inflammatory Th1 and Th17 cytokines including IL-6, IL-12p70, IL-17AF, and IL-22 yet increased levels of immunosuppressive cytokine indoleamine 2,3-dioxygenase. IFN-Exo could also induce Tregs in vitro in a murine splenocyte culture, likely mediated by a third-party accessory cell type. Further, IFN-Exo characterization by deep RNA sequencing suggested that IFN-Exo contains anti-inflammatory RNAs, where their inactivation partially hindered the exosomes potential to induce Tregs. Furthermore, we found that IFN-Exo harbors multiple anti-inflammatory and neuroprotective proteins. These results not only shed light on stem cell therapeutic mechanisms but also provide evidence that MSC-derived exosomes can potentially serve as cell-free therapies in creating a tolerogenic immune response to treat autoimmune and central nervous system disorders. © 2019 American Chemical Society.

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