Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

The SH3 domains of the pr… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

The SH3 domains of the protein kinases ITK and LCK compete for adjacent sites on T cell?specific adapter protein

Artikel i vetenskaplig tidskrift
Författare T. C. B. Andersen
P. E. Kristiansen
Z. Huszenicza
Maria U Johansson
R. P. Gopalakrishnan
H. Kjelstrup
S. Boyken
V. Sundvold-Gjerstad
S. Granum
M. Sorlie
P. H. Backe
D. B. Fulton
B Göran Karlsson
A. H. Andreotti
A. Spurkland
Publicerad i Journal of Biological Chemistry
Volym 294
Nummer/häfte 42
Sidor 15480-15494
ISSN 0021-9258
Publiceringsår 2019
Publicerad vid Svenskt NMR-centrum vid Göteborgs universitet
Sidor 15480-15494
Språk en
Länkar dx.doi.org/10.1074/jbc.RA119.008318
Ämnesord Src homology 3 domain (SH3 domain), adaptor protein, T-cell, protein, phosphorylation, protein structure, protein-protein interaction, protein, kinase, cell signaling, NMR, nuclear magnetic resonance, tyrosine-protein kinase, LCK proto-oncogene SRC family tyrosine kinase, IL-2-inducible T cell kinase (ITK), T cell-specific adapter protein, (TSAD), immunity, cell-activation, recognition domains, negative regulation, structural, basis, tyrosine kinase, chemical-shift, high-affinity, binding, peptide, specificity, Biochemistry & Molecular Biology
Ämneskategorier Immunologi inom det medicinska området

Sammanfattning

T-cell activation requires stimulation of specific intracellular signaling pathways in which protein-tyrosine kinases, phosphatases, and adapter proteins interact to transmit signals from the T-cell receptor to the nucleus. Interactions of LCK proto-oncogene, SRC family tyrosine kinase (LCK), and the IL-2?inducible T cell kinase (ITK) with the T cell-specific adapter protein (TSAD) promotes LCK-mediated phosphorylation and thereby ITK activation. Both ITK and LCK interact with TSAD's proline-rich region (PRR) through their Src homology 3 (SH3) domains. Whereas LCK may also interact with TSAD through its SH2 domain, ITK interacts with TSAD only through its SH3 domain. To begin to understand on a molecular level how the LCK SH3 and ITK SH3 domains interact with TSAD in human HEK293T cells, here we combined biochemical analyses with NMR spectroscopy. We found that the ITK and LCK SH3 domains potentially have adjacent and overlapping binding sites within the TSAD PRR amino acids (aa) 239?274. Pulldown experiments and NMR spectroscopy revealed that both domains may bind to TSAD aa 239?256 and aa 257?274. Co-immunoprecipitation experiments further revealed that both domains may also bind simultaneously to TSAD aa 242?268. Accordingly, NMR spectroscopy indicated that the SH3 domains may compete for these two adjacent binding sites. We propose that once the associations of ITK and LCK with TSAD promote the ITK and LCK interaction, the interactions among TSAD, ITK, and LCK are dynamically altered by ITK phosphorylation status.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?