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Gene profiling reveals increased expression of uteroglobin and other anti-inflammatory genes in glucocorticoid-treated nasal polyps.

Artikel i vetenskaplig tidskrift
Författare Mikael Benson
Lena M S Carlsson
Mikael Adner
Margareta Jernås
Mats Rudemo
Anders Sjögren
Per-Arne Svensson
Rolf Uddman
Lars Olaf Cardell
Publicerad i Journal of Allergy and Clinical Immunology
Volym 113
Nummer/häfte 6
Sidor 1137-43
ISSN 0091-6749
Publiceringsår 2004
Publicerad vid Institutionen för matematisk statistik
Institutionen för kvinnors och barns hälsa, Avdelningen för pediatrik
Institutionen för invärtesmedicin, Avdelningen för kroppssammansättning och metabolism
Sidor 1137-43
Språk en
Ämneskategorier Medicin och Hälsovetenskap

Sammanfattning

BACKGROUND: Treatment with local glucocorticoids (GCs) decreases symptoms and the size of nasal polyps. This might depend on the downregulation of proinflammatory genes, as well as the upregulation of anti-inflammatory genes. OBJECTIVE: We sought to identify GC-regulated anti-inflammatory genes in nasal polyps. METHODS: Affymetrix DNA microarrays were used to analyze the expression of 22,283 genes in 4 nasal polyps before and after local treatment with fluticasone (400 microg/d). Expression of uteroglobin and mammaglobin B was analyzed with real-time PCR in 6 nasal polyps and in nasal biopsy specimens from 6 healthy control subjects. RESULTS: Two hundred three genes had changed in expression in treated polyps, and 139 had known functions: 54 genes were downregulated, and 85 were upregulated. Genes associated with inflammation constituted the largest single functional group. These genes affected key steps in inflammation (eg, immunoglobulin production; antigen processing and presentation; and the chemoattraction and activation of granulocytes, T cells, and B cells). Several proinflammatory genes were downregulated. In contrast, some anti-inflammatory genes were upregulated. The gene that increased most in terms of expression was uteroglobin. This was confirmed with real-time PCR. By contrast, expression of uteroglobin was lower in untreated polyps than in healthy nasal mucosa. Immunohistochemical investigation showed staining of uteroglobin in the epithelium and in seromucous glands in control subjects and in nasal polyps. CONCLUSION: Upregulation of anti-inflammatory genes, such as uteroglobin, might contribute to the effects of local treatment with GCs in nasal polyps.

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