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Discrimination of human tumor radioresponsiveness using low-dose rate irradiation

Artikel i vetenskaplig tidskrift
Författare Thomas Björk-Eriksson
C. West
E. Karlsson
Claes Mercke
Publicerad i Int J Radiat Oncol Biol Phys
Volym 42
Nummer/häfte 5
Sidor 1147-53
ISSN 0360-3016 (Print)
Publiceringsår 1998
Publicerad vid Institutionen för särskilda specialiteter, Avdelningen för onkologi
Sidor 1147-53
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Cell Survival/radiation effects, Humans, Models, Biological, Radiation Oncology/*methods, *Radiation Tolerance, Radiotherapy Dosage, Tumor Cells, Cultured/radiation effects
Ämneskategorier Cancer och onkologi

Sammanfattning

PURPOSE: Evaluation of the theoretical and practical value of using low-dose rate (LDR) irradiation to increase the resolution of radiosensitivity testing of primary human tumors using clonogenic assays. METHODS AND MATERIALS: Fourteen human tumor cell lines were assessed for surviving fraction at 2-8 Gy (SF2-SF8) using low-dose rate irradiation and a clonogenic assay. Further data were collected from the literature for 64 low-dose rate irradiation survival curves from human tumor cell lines. The data were grouped into five different radioresponsiveness categories (A-E). An analysis was made of the ability of the graded survival levels to discriminate between the different radioresponse groups and compared with previous analyses for high-dose rate SF2. Fifteen human cervical carcinoma specimens were analysed for SF2 and SF3.5 following high- and low-dose rate irradiation. RESULTS: Low-dose rate irradiation increased the spread of tumor cell line radiosensitivity data and the ability to discriminate between radioresponse groups was greater at low than at high-dose rates. Using low-dose rate irradiation on primary tumor specimens and a soft agar clonogenic assay decreased the success rate in obtaining data. The latter dropped from 70% for high-dose rate SF2 to 51% for low-dose rate SF3.5. CONCLUSIONS: The work on cell lines illustrates that low-dose rate irradiation does improve the ability of clonogenic radiosensitivity measurements to discriminate between tumors of different radioresponsiveness groups. However, using low-dose rate irradiation on primary human tumors with a soft agar clonogenic assay was not practical because of reducing the success rate for obtaining data for radiosensitivity measurements.

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