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Calcium-dependent proteolysis in rabbit lens epithelium after oxidative stress

Artikel i vetenskaplig tidskrift
Författare Madeleine Andersson
Johan Sjöstrand
Anne Petersen
Jan-Olof Karlsson
Publicerad i Ophthalmic Res
Volym 30
Nummer/häfte 3
Sidor 157-67
Publiceringsår 1998
Publicerad vid Institutionen för klinisk neurovetenskap, Sektionen för oftalmologi
Institutionen för anatomi och cellbiologi
Sidor 157-67
Språk en
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Ämnesord Animal, Calcium/*pharmacology, Calpain/*metabolism, Cataract/chemically induced/enzymology/pathology, Epithelium/drug effects/enzymology/pathology, Female, Hydrogen Peroxide/toxicity, Lens, Crystalline/*drug effects/enzymology/pathology, Organ Culture, *Oxidative Stress, Rabbits, Support, Non-U.S. Gov't
Ämneskategorier Cellbiologi

Sammanfattning

The purpose of this study was to examine changes in calcium-dependent proteolytic activity in the lens epithelium from whole rabbit lenses exposed to long-term oxidative stress at near physiological levels. Rabbit lenses, incubated in 50 microM H2O2 for 1 or 24 h, were checked for clarity and morphological changes in the epithelium. Proteolytic activity was measured in the epithelium using a fluorogenic synthetic substrate; N-succinyl-Leu-Tyr-7-amino-4-methylocoumarin, both in the presence and the absence of calcium (1 mM Ca2+ and 5 mM EDTA respectively). The effect on transparency and morphology of the epithelium following a 1-hour incubation in 100 microM H2O2 was also studied. Lenses incubated in 50 microM H2O2 were clear even after 24h. After a 1-hour incubation in 50 microM H2O2 the epithelium of the exposed lens appeared normal. However, after 24 h the epithelium cells appeared swollen and microscopical examination showed extensive intracellular and subepithelial vacuolization. Incubation in 100 microM H2O2 for 1 h caused loss of transparency; vacuole formation, globulization of the superficial lens fibers and death of the epithelial cells. There was a 55% increase in calcium-dependent proteolytic activity after 1 h in 50 microM H2O2, implying a role for the calcium-activated protease calpain in oxidatively induced cataract.

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