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- Kristina Hedfalk
Kristina Hedfalk
Professor
Institutionen för kemi och molekylärbiologiProprefekt
Svenskt NMR-centrum vid GUProprefekt
Institutionen för kemi och molekylärbiologiOm Kristina Hedfalk
Forskning
Min forskning riktar sig mot membranbundna proteiners struktur, funktion och reglering med ett särskilt fokus på aquaporiner; vattenkanalar i cellmembranet hos allt levande. Ett stort intresse finns också för att uppnå en ökad kunskap om produktionen av membranbundna proteiner i syfte att minimera den välkända "flaskhalsen" för detaljstudier av eukaryota membranproteiner i synnerhet.
Forskningsgrupp: Johannes Panagiotidis (doktorand), Ulrika Lind (1:e forskningsingenjör) & Nicklas Bonander (1:e forskningsingenjör)
Citeringar: https://scholar.google.se/citations?user=_gE3NHsAAAAJ&hl=sv
Undervisning
Jag undervisar i biokemi, avancerade nivå:
- Design och produktion av biomolekyler (KEM350), kursansvarig
Uppdrag
- Proprefekt vid institutionen
Protein Production Sweden - PPS
Hedfalks lab utgör en lokal nod i Protein Production Sweden (PPS; www.gu.se/pps), en nationell forskningsinfrastruktur etablerad 2022. PPS fokuserar på produktion och rening av (huvudsakligen) rekombinanta proteiner för alla svenska forskare. De sju ingående PPS laboratorierna använder flera olika experssionssystem vilka tillåter produktion av många olika typer av proteiner. Forskare når PPS infrastrukturen via en gemensam portal och erhåller individuellt stöd baserat på deras forskningsbehov genom hela processen för protein produktion och rening. Detta kan inkludera alla steg från rådgivning till initial design till slutlig kvalitetskontroll, eller enskilda eller flera steg av processen.
För att få mer information om PPS' service och ingående noder, eller för att ansöka om stöd för projekt, besök:
Kontakta gärna ansvarig för noden för protein produktion i jäst: Kristina Hedfalk (kristina. hedfalk@gu.se)
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High-resolution structure of a fish aquaporin reveals a novel extracellular
fold
Jiao Zeng, Florian Schmitz, Simon Isaksson, Jessica Glas, Olivia Arbab, Martin Andersson, Kristina Sundell, Leif A Eriksson, Kunchithapadam Swaminathan, Susanna Törnroth-Horsefield, Kristina Hedfalk
Life Science Alliance - 2022 -
Yeast as a tool for membrane protein production and structure
determination
Antonio Carlesso, Raquel Delgado, Oriol Ruiz Isant, Owens Uwangue, D. Valli, R. M. Bill, Kristina Hedfalk
Fems Yeast Research - 2022 -
LPIAT1/MBOAT7 contains a catalytic dyad transferring polyunsaturated fatty acids to
lysophosphatidylinositol.
Andrea Caddeo, Kristina Hedfalk, Stefano Romeo, Piero Pingitore
Biochimica et biophysica acta. Molecular and cell biology of lipids - 2021 -
Molecular Level Characterization of Circulating Aquaporin-4 Antibodies in Neuromyelitis Optica Spectrum
Disorder
J. Li, S. A. Bazzi, Florian Schmitz, H. Tanno, J. R. McDaniel, C. H. Lee, C. Joshi, J. E. Kim, N. Monson, B. M. Greenberg, Kristina Hedfalk, E. Melamed, G. C. Ippolito
Neurology(R) neuroimmunology & neuroinflammation - 2021 -
A bimolecular fluorescence complementation flow cytometry screen for membrane protein
interactions
Florian Schmitz, Jessica Glas, Richard Neutze, Kristina Hedfalk
Scientific Reports - 2021 -
Aquaporins - Expression, purification and
characterization
R. M. Bill, Kristina Hedfalk
Biochimica Et Biophysica Acta-Biomembranes - 2021 -
Quantitative analysis of H2O2 transport through purified membrane
proteins
Hao Wang, Stefan Schoebel, Florian Schmitz, H. Dong, Kristina Hedfalk
MethodsX - 2020 -
Characterization of aquaporin-driven hydrogen peroxide
transport.
Hao Wang, Stefan Schoebel, Florian Schmitz, Hansong Dong, Kristina Hedfalk
Biochimica et biophysica acta. Biomembranes - 2020 -
Cys Site-Directed Mutagenesis of the Human SLC1A5 (ASCT2) Transporter: Structure/Function Relationships and Crucial Role of Cys467 for Redox Sensing and Glutamine
Transport
M. Scalise, L. Pochini, L. Console, G. Pappacoda, Piero Pingitore, Kristina Hedfalk, C. Indiveri
International Journal of Molecular Sciences - 2018 -
Protein-Containing Lipid Bilayers Intercalated with Size-Matched Mesoporous Silica Thin
Films
Simon Isaksson, Erik B. Watkins, Kathryn L. Browning, Tania Kjellerup Lind, Marité Cárdenas, Kristina Hedfalk, Fredrik Höök, Martin Andersson
Nano letters - 2017 -
Analysis of aquaporins from the euryhaline barnacle Balanus improvisus reveals differential expression in response to changes in
salinity
Ulrika Lind, Michael Järvå, Magnus Alm Rosenblad, Piero Pingitore, Emil Karlsson, Anna Lisa Wrange, Emelie Kamdal, Kristina Sundell, Carl André, Per R. Jonsson, Jonathan N. Havenhand, Leif A Eriksson, Kristina Hedfalk, Anders Blomberg
PLoS ONE - 2017 -
Applying bimolecular fluorescence complementation to screen and purify aquaporin protein:protein
complexes.
Jennie Sjöhamn, Petra Båth, Richard Neutze, Kristina Hedfalk
Protein science : a publication of the Protein Society - 2016 -
Plasma Membrane Abundance of Human Aquaporin 5 Is Dynamically Regulated by Multiple
Pathways.
Philip Kitchen, Fredrik Öberg, Jennie Sjöhamn, Kristina Hedfalk, Roslyn M Bill, Alex C Conner, Matthew T Conner, Susanna Törnroth-Horsefield
PloS one - 2015 -
Cysteine is not a substrate but a specific modulator of human ASCT2 (SLC1A5)
transporter.
Mariafrancesca Scalise, Lorena Pochini, Piero Pingitore, Kristina Hedfalk, Cesare Indiveri
FEBS letters - 2015 -
Low CO2 permeability of cholesterol-containing liposomes detected by stopped-flow fluorescence
spectroscopy
G. Tsiavaliaris, F. Itel, Kristina Hedfalk, S. Al-Samir, W. Meier, G. Gros, V. Endeward
Faseb Journal - 2015 -
X-ray structure of human aquaporin 2 and its implications for nephrogenic diabetes insipidus and
trafficking.
Anna Frick, Urszula Kosinska-Eriksson, Fabrizio de Mattia, Fredrik Öberg, Kristina Hedfalk, Richard Neutze, Willem J de Grip, Peter M T Deen, Susanna Törnroth-Horsefield
Proceedings of the National Academy of Sciences of the United States of America - 2014 -
Monitoring the osmotic response of single yeast cells through force measurement in the environmental scanning electron
microscope
Anna Jansson, Alexandra Nafari, Kristina Hedfalk, Eva Olsson, K. Svensson, Anke Sanz-Velasco
Measurement science and technology - 2014 -
Recombinant PNPLA3 protein shows triglyceride hydrolase activity and its I148M mutation results in loss of
function.
Piero Pingitore, Carlo Pirazzi, Rosellina Margherita Mancina, Benedetta Maria Motta, Cesare Indiveri, Arturo Pujia, Tiziana Montalcini, Kristina Hedfalk, Stefano Romeo
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - 2014 -
Transport mechanism and regulatory properties of the human amino acid transporter ASCT2
(SLC1A5).
Mariafrancesca Scalise, Lorena Pochini, Simona Panni, Piero Pingitore, Kristina Hedfalk, Cesare Indiveri
Amino acids - 2014 -
PNPLA3 has retinyl-palmitate lipase activity in human hepatic stellate
cells
Carlo Pirazzi, L. Valenti, Benedetta Maria Motta, Piero Pingitore, Kristina Hedfalk, Rosellina Margherita Mancina, Maria Antonella Burza, C. Indiveri, Y. Ferro, T. Montalcini, Cristina Maglio, P. Dongiovanni, S. Fargion, R. Rametta, A. Pujia, Linda Andersson, Saswati Ghosal, Malin Levin, Olov Wiklund, M. Iacovino, Jan Borén, Stefano Romeo
Human Molecular Genetics - 2014 -
Unraveling aquaporin interaction
partners.
Jennie Sjöhamn, Kristina Hedfalk
Biochimica et Biophysica Acta (BBA) General Subjects - 2014 -
Further advances in the production of membrane proteins in Pichia
pastoris.
Kristina Hedfalk
Bioengineered - 2013 -
In vivo studies of aquaporins 3 and 10 in human stratum
corneum.
Jakob Mutanu Jungersted, Julie Bomholt, Niada Bajraktari, Jesper Søndergaard Hansen, Dan A Klærke, Per Amstrup Pedersen, Kristina Hedfalk, Kent Høier Nielsen, Tove Agner, Claus Hélix-Nielsen
Archives of dermatological research - 2013 -
Large scale production of the active human ASCT2 (SLC1A5) transporter in Pichia pastoris - functional and kinetic asymmetry revealed in
proteoliposomes
Piero Pingitore, L. Pochini, M. Scalise, M. Galluccio, Kristina Hedfalk, C. Indiveri
Biochimica Et Biophysica Acta-Biomembranes - 2013 -
Recombinant production of the human aquaporins in the yeast Pichia pastoris (Invited
Review).
Kristina Hedfalk, Fredrik Öberg
Molecular membrane biology - 2013 -
Codon optimisation for heterologous gene expression in
yeast.
Kristina Hedfalk
Recombinant Protein Production in Yeast / ed. by Roslyn M. Bill - 2012 -
CO2 permeability of cell membranes is regulated by membrane cholesterol and protein gas
channels
F. Itel, S. Al-Samir, Fredrik Öberg, M. Chami, M. Kumar, C. T. Supuran, P. M. T. Deen, W. Meier, Kristina Hedfalk, G. Gros, V. Endeward
Faseb Journal - 2012 -
Glycosylation increases the thermostability of human aquaporin 10
protein.
Fredrik Öberg, Jennie Sjöhamn, Gerhard Fischer, Anders Pedersen, Richard Neutze, Kristina Hedfalk
The Journal of biological chemistry - 2011 -
Improving recombinant eukaryotic membrane protein yields in Pichia pastoris: the importance of codon optimization and clone
selection.
Fredrik Öberg, Jennie Sjöhamn, Matthew T Conner, Roslyn M. Bill, Kristina Hedfalk
Molecular membrane biology - 2011 -
Increasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield: the use of a respiratory strain as a microbial cell
factory
Cecilia Ferndahl, N. Bonander, C. Logez, R. Wagner, Lena Gustafsson, Christer Larsson, Kristina Hedfalk, R. A. J. Darby, R. M. Bill
Microbial Cell Factories - 2010 -
Structural insights into eukaryotic aquaporin
regulation.
Susanna Törnroth-Horsefield, Kristina Hedfalk, Gerhard Fischer, Karin Lindkvist-Petersson, Richard Neutze
FEBS letters - 2010 -
Structural and functional analysis of SoPIP2;1 mutants adds insight into plant aquaporin
gating.
Maria Nyblom, Anna Frick, Yi Wang, Mikael Ekvall, Karin Hallgren, Kristina Hedfalk, Richard Neutze, Emad Tajkhorshid, Susanna Törnroth-Horsefield
Journal of molecular biology - 2009 -
Crystal structure of a yeast aquaporin at 1.15 angstrom reveals a novel gating
mechanism.
Gerhard Fischer, Urszula Kosinska-Eriksson, Camilo Aponte-Santamaría, Madelene Palmgren, Cecilia Geijer, Kristina Hedfalk, Stefan Hohmann, Bert L de Groot, Richard Neutze, Karin Lindkvist-Petersson
PLoS biology - 2009 -
Insight into factors directing high production of eukaryotic membrane proteins; production of 13 human AQPs in Pichia
pastoris.
Fredrik Öberg, Mikael Ekvall, Maria Nyblom, Anna Backmark, Richard Neutze, Kristina Hedfalk
Molecular membrane biology - 2009 -
Affinity tags can reduce merohedral twinning of membrane protein
crystals
Anna Backmark, Maria Nyblom, Susanna Törnroth-Horsefield, Urszula Kosinska-Eriksson, Kristina Hedfalk, Karin Lindkvist-Petersson, Richard Neutze, Rob Horsefield
Acta Crystallogarphis D - 2008 -
Production, characterization and crystallization of the Plasmodium falciparum
aquaporin.
Kristina Hedfalk, Nina Pettersson, Fredrik Oberg, Stefan Hohmann, Euan Gordon
Protein expression and purification - 2008 -
Exceptional overproduction of a functional human membrane
protein
Maria Nyblom, Fredrik Öberg, Karin Lindkvist-Petersson, Karin Hallgren, H. Findlay, J. Wikstrom, A. Karlsson, Örjan Hansson, P. J. Booth, Roslyn M. Bill, Richard Neutze, Kristina Hedfalk
Protein Expression and Purification - 2007 -
Improved yields of full-length functional human FGF1 can be achieved using the methylotrophic yeast <em>Pichia
pastoris</em>
A Fantoni, RM Bill, Lena Gustafsson, Kristina Hedfalk
Protein Expression and Purification - 2007 -
Aquaporin
gating
Susanna Törnroth-Horsefield, Kristina Hedfalk, M. Karlsson, U. Johanson, Y. Wang, E. Tajkhorshid, Rob Horsefield, M. Nyblom, A. Backmark, P. Kjellbom, Richard Neutze
Journal of Biomolecular Structure & Dynamics - 2007 -
Structural mechanism of plant aquaporin
gating
Susanna Törnroth-Horsefield, Y Wang, Kristina Hedfalk, U Johanson, M Karlsson, E Tajkhorshid, Richard Neutze, P Kjellbom
Nature - 2006 -
Eukaryotic expression: developments for structural
proteomics.
A R Aricescu, R Assenberg, R M Bill, D Busso, V T Chang, S J Davis, A Dubrovsky, Lena Gustafsson, Kristina Hedfalk, U Heinemann, I M Jones, D Ksiazek, C Lang, K Maskos, A Messerschmidt, S Macieira, Y Peleg, A Perrakis, A Poterszman, G Schneider, T K Sixma, J L Sussman, G Sutton, N Tarboureich, T Zeev-Ben-Mordehai, E Yvonne Jones
Acta crystallographica. Section D, Biological crystallography - 2006 -
Aquaporin
gating
Kristina Hedfalk, Susanna Törnroth-Horsefield, Maria Nyblom, U Johanson, P Kjellbom, Richard Neutze
Current Opinion in Structural Biology - 2006 -
Eukaryotic expression: developments for structural
proteomics
AR Aricescu, R Assenberg, RM Bill, D Busso, VT Chang, SJ Davis, A Dubrovsky, Lena Gustafsson, Kristina Hedfalk, et al et al.
Acta Crystallogr D Biol Crystallogr - 2006 -
Design of improved membrane protein production experiments: Quantitation of the host
response
Nicklas Bonander, Kristina Hedfalk, Christer Larsson, Petter Mostad, Cecilia Chang, Lena Gustafsson, Roslyn Bill
Protein Science - 2005 -
Engineering of a novel Saccharomyces cerevisiae wine strain with a respiratory phenotype at high external glucose
concentrations
Cecilia Henricsson, Maria de Jesus-Ferreira, Kristina Hedfalk, Karin Elbing, Christer Larsson, Joakim Norbeck, Roslyn Bill, Stefan Hohmann, Lena Gustafsson
Applied and Environmental Microbiology - 2005 -
Role of Fps1 hydrophilic extensions and identification of residues controlling glycerol
transport
Caroline Filipsson, Kristina Hedfalk, Sara Karlgren, J. G. Mullins, Roslyn M. Bill, Stefan Hohmann, Jan Rydström
Febs Journal - 2005 -
A Regulatory Domain in the C-terminal Extension of the Yeast Glycerol Channel
Fps1p
Kristina Hedfalk, Roslyn M. Bill, Johathan G.L. Mullins, Sara Karlgren, Caroline Filipsson, Johanna Bergström, Markus J. Tamás, Jan Rydström, Stefan Hohmann
Journal of biological chemistry - 2004 -
Role of Fps1 hydrophilic extensions and identification of residues controlling glycerol
transport
Caroline Filipsson, Kristina Hedfalk, Sara Karlgren, J. G. L Mullins, Roslyn M. Bill, Stefan Hohmann, Jan Rydström
Biochimica Et Biophysica Acta-Bioenergetics - 2004 -
Structural studies on PM28A, an aquaporin from
spinach
Susanna Törnroth, M Karlsson, Kristina Hedfalk, P Kjellbom, Richard Neutze
Biochim Biophys Acta-Bioenergetics - 2004 -
Reconstitution of water channel function of an aquaporin overexpressed and purified from Pichia
pastoris.
Maria Karlsson, Dimitrios Fotiadis, Sara Sjövall, Ingela Johansson, Kristina Hedfalk, Andreas Engel, Per Kjellbom
FEBS letters - 2003 -
A Short Regulatory Domain Restricts Glycerol Transport through Yeast
Fps1p
Markus J. Tamás, Sara Karlgren, Roslyn M. Bill, Kristina Hedfalk, L Allegri, Marie Ferreira, J.M. Thevelein, Jan Rydström, J. G. L Mullins, Stefan Hohmann
J. Biol. Chem - 2003 -
Structure, Function and Regulation of Fps1p. A Eukaryotic Solute Efflux
Channel
Kristina Hedfalk
2002 -
Analysis of the pore of the unusual major intrinsic protein channel, yeast
Fps1p.
Roslyn M. Bill, Kristina Hedfalk, Sara Karlgren, J G Mullins, J Rydström, Stefan Hohmann
The Journal of biological chemistry - 2001